Rabbit anti-Human, Mouse POLR2J1 Polyclonal Antibody | anti-POLR2J1 antibody

POLR2J1 Polyclonal Antibody

Cat. Num. AAA3009957

• Gene Names: POLR2J; RPB11; RPB11A; RPB11m; hRPB14; POLR2J1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: POLR2J1; Polyclonal Antibody; POLR2J1 Polyclonal Antibody; DNA-directed RNA polymerase II subunit RPB11-a; RNA polymerase II subunit B11-a; RPB11a; DNA-directed RNA polymerase II subunit J-1; RNA polymerase II 13.3 kDa subunit; POLR2J; anti-POLR2J1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: POLR2J1 polyclonal antibody detects endogenous levels of POLR2J1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 117

• Applicable Applications for anti-POLR2J1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant full length Human POLR2J1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of POLR2J1 polyclonal antibody)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells, using POLR2J1 polyclonal antibody)

Related Product Information for anti-POLR2J1 antibody

Three genes (POLR2 J1, POLR2 J2, and POLR2 J3) form a cluster of total length of 214 530 bp in the genetic locus 7q22.1 on the long arm of chromosome 7 (contig NT_007933). The fourth gene (POLR2 J4, 31 040 bp) was localized in the cytogenetic locus 7p13 of the short arm of chromosome 7 (contig NT_007819). An analysis enabled us to refine dissimilar experimental data on the mapping of the hRPB11 subunit gene on chromosome 7. In particular, the presence of three sites of its localization according to data on hybridization with fluorescent-labeled probes (the FISH method) was explained. It was established that, upon the expression of the four human POLR2 J genes, at least 14 types of mature mRNAs encoding somewhat differing hRPB11 isoforms can be synthesized. Eleven of these mRNAs were revealed (as full-length copies or clearly identifiable fragments) in the available databases of expressed sequence tags and cDNAs. The most probable scheme of origination of the multiple genes of the POLR2 J family as a result of three consecutive segmented duplications increasing in size was proposed and substantiated. On the basis of the scheme, some assumptions on the pathways of evolution of separate human genes and the mechanisms of generation of protein diversity in higher eukaryotes were made

NCBI and Uniprot Product Information

• NCBI GI #: 5453932
• NCBI GeneID: 5439
• NCBI Accession #: NP_006225.1
• NCBI GenBank Nucleotide #: NP_006225.1
• UniProt Accession #: P52435
• Molecular Weight: ~ 13kDa
• NCBI Official Full Name: DNA-directed RNA polymerase II subunit RPB11-a isoform 1
• NCBI Official Synonym Full Names: RNA polymerase II subunit J
• NCBI Official Symbol: POLR2J
• NCBI Official Synonym Symbols: RPB11; RPB11A; RPB11m; hRPB14; POLR2J1
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB11-a
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB11-a
• UniProt Gene Name: POLR2J
• UniProt Synonym Gene Names: POLR2J1; RNA polymerase II subunit B11-a; RPB11a
• UniProt Entry Name: RPB11_HUMAN

NCBI Description

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. [provided by RefSeq, Jul 2008]

Uniprot Description

POLR2J: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft. Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.

Protein type: RNA splicing; RNA processing; Transferase; DNA-binding

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: nucleoplasm; DNA-directed RNA polymerase II, core complex; nucleus

Molecular Function: protein dimerization activity; protein binding; DNA binding; DNA-directed RNA polymerase activity; LRR domain binding

Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; nucleotide-excision repair; somatic stem cell maintenance; positive regulation of viral transcription; transcription-coupled nucleotide-excision repair; RNA splicing; RNA elongation from RNA polymerase II promoter; gene expression; DNA repair