Rabbit POLR2C Polyclonal Antibody | anti-POLR2C antibody

Anti-POLR2C Antibody

Cat. Num. AAA416380

• Gene Names: POLR2C; RPB3; RPB31; hRPB33; hsRPB3
• Reactivity: Human. Predicted: Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: POLR2C; Polyclonal Antibody; Anti-POLR2C Antibody; anti-POLR2C antibody

• Host: Rabbit
• Reactivity: Human. Predicted: Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 1422

• Applicable Applications for anti-POLR2C antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA titer: 1:20,000-1:80,000; Western blot analysis: 1:500-1:1,000; Immunohistochemistry: 1:100-1:500; Optimal dilutions/concentrations should be determined by the end user
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Rabbit polyclonal POLR2C (1) antibody was raised against a recombinated human POLR2C protein 38-238aa (BC000409).
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-POLR2C antibody

This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21. [provided by RefSeq, Jul 2008]

References

(1) Oufattole,M., Lin,S.W., Liu,B., et al. Ribonucleic acid polymerase II binding subunit 3 (Rpb3), a potential nuclear target of insulin-like growth factor binding protein-3. Endocrinology 147 (5), 2138-2146 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 38197145
• NCBI GeneID: 5432
• Molecular Weight: 31,441 Da
• NCBI Official Full Name: Homo sapiens polymerase (RNA) II (DNA directed) polypeptide C, 33kDa, mRNA
• NCBI Official Synonym Full Names: polymerase (RNA) II (DNA directed) polypeptide C, 33kDa
• NCBI Official Symbol: POLR2C
• NCBI Official Synonym Symbols: RPB3; RPB31; hRPB33; hsRPB3
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB3; DNA-directed RNA polymerase II 33 kDa polypeptide; DNA-directed RNA polymerase II subunit C; RNA polymerase II subunit 3; RNA polymerase II subunit B3; RPB33
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB3
• UniProt Gene Name: POLR2C
• UniProt Entry Name: RPB3_HUMAN

NCBI Description

This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21. [provided by RefSeq, Jul 2008]

Uniprot Description

POLR2C: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft. Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family.

Protein type: Transcription initiation complex; Nucleotide Metabolism - purine; DNA-binding; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 16q13-q21

Cellular Component: microtubule cytoskeleton; nucleoplasm; cytoplasm; DNA-directed RNA polymerase II, core complex; nucleus

Molecular Function: protein dimerization activity; DNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; nucleotide-excision repair; somatic stem cell maintenance; positive regulation of viral transcription; RNA splicing; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; gene expression; DNA repair