Rabbit POLR2A Polyclonal Antibody | anti-POLR2A antibody

Rabbit anti-Cricetulus griseus (Chinese hamster)(Cricetulus barabensis griseus) POLR2A Polyclonal Antibody

• Gene Names: Polr2a; Rpb1
• Reactivity: Cricetulus griseus (Chinese hamster)(Cricetulus barabensis griseus)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: POLR2A; Polyclonal Antibody; Rabbit anti-Cricetulus griseus (Chinese hamster)(Cricetulus barabensis griseus) POLR2A Polyclonal Antibody; POLR2A Antibody; DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; EC 2.7.7.6; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; anti-POLR2A antibody

• Host: Rabbit
• Reactivity: Cricetulus griseus (Chinese hamster)(Cricetulus barabensis griseus)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-POLR2A antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) POLR2A protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 345842509
• NCBI GeneID: 100689016
• NCBI Accession #: NP_001230931.1
• NCBI GenBank Nucleotide #: NM_001244002.1
• UniProt Accession #: P11414; Q76M93
• Molecular Weight: 217,206 Da
• NCBI Official Full Name: DNA-directed RNA polymerase II subunit RPB1
• NCBI Official Symbol: Polr2a
• NCBI Official Synonym Symbols: Rpb1
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB1; LOW QUALITY PROTEIN: DNA-directed RNA polymerase II subunit RPB1
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB1
• UniProt Gene Name: POLR2A
• UniProt Synonym Gene Names: RNA polymerase II subunit B1

Uniprot Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing (). Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (). Methylation and dimethylation have a repressive effect on target genes expression ().