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Western Blot (WB) (Jurkat cells were subjected to SDS PAGE followed by western blot with MBS7602852 (POLR1E antibody) at dilution of 1:500)

Rabbit POLR1E Polyclonal Antibody | anti-POLR1E antibody

POLR1E Rabbit Polyclonal

Gene Names
POLR1E; PAF53; PRAF1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
>=95% as determined by SDS-PAGE
Immunogen Affinity Purified
Synonyms
POLR1E; Polyclonal Antibody; POLR1E Rabbit Polyclonal; anti-POLR1E antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>=95% as determined by SDS-PAGE
Immunogen Affinity Purified
Form/Format
Liquid
Sequence Length
481
Applicable Applications for anti-POLR1E antibody
ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
Immunogen
Polymerase (RNA) I polypeptide E, 53kDa
Preparation and Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Western Blot (WB)

(Jurkat cells were subjected to SDS PAGE followed by western blot with MBS7602852 (POLR1E antibody) at dilution of 1:500)

Western Blot (WB) (Jurkat cells were subjected to SDS PAGE followed by western blot with MBS7602852 (POLR1E antibody) at dilution of 1:500)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,260 Da
NCBI Official Full Name
DNA-directed RNA polymerase I subunit RPA49 isoform X1
NCBI Official Synonym Full Names
polymerase (RNA) I subunit E
NCBI Official Symbol
POLR1E
NCBI Official Synonym Symbols
PAF53; PRAF1
NCBI Protein Information
DNA-directed RNA polymerase I subunit RPA49
UniProt Protein Name
DNA-directed RNA polymerase I subunit RPA49
UniProt Gene Name
POLR1E
UniProt Synonym Gene Names
PAF53; PRAF1; RNA polymerase I subunit A49
UniProt Entry Name
RPA49_HUMAN

Uniprot Description

POLR1E: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF. Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 9p13.2

Cellular Component: DNA-directed RNA polymerase I complex; nucleolus; nucleoplasm; nucleus

Molecular Function: DNA binding

Biological Process: positive regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; rRNA transcription; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter

Research Articles on POLR1E

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Product Notes

The POLR1E polr1e (Catalog #AAA7602852) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POLR1E Rabbit Polyclonal reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's POLR1E can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the POLR1E polr1e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLR1E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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