Rat Glutathione Peroxidase 1 (GPX1) ELISA Kit | GPX1 elisa kit

Rat Glutathione Peroxidase 1 (GPX1) ELISA Kit

Cat. Num. AAA2021740

• Gene Names: GPX1; GPXD; GSHPX1
• Reactivity: Rat
• Synonyms: Glutathione Peroxidase 1 (GPX1); Rat Glutathione Peroxidase 1 (GPX1) ELISA Kit; GSHPX1; Cellular glutathione peroxidase; GPX1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutathione Peroxidase 1 (GPX1).; No significant cross-reactivity or interference between Glutathione Peroxidase 1 (GPX1) and analogues was observed.
• Sequence Length: 98

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 7.5pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione Peroxidase 1 (GPX1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione Peroxidase 1 (GPX1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GPX1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Peroxidase 1 (GPX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione Peroxidase 1 (GPX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Peroxidase 1 (GPX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione Peroxidase 1 (GPX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GPX1 elisa kit

Enzyme & Kinase; Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 311033481
• NCBI GeneID: 2876
• Molecular Weight: 10,289 Da
• NCBI Official Full Name: Glutathione peroxidase 1
• NCBI Official Synonym Full Names: glutathione peroxidase 1
• NCBI Official Symbol: GPX1
• NCBI Official Synonym Symbols: GPXD; GSHPX1
• NCBI Protein Information: glutathione peroxidase 1
• UniProt Protein Name: Glutathione peroxidase 1
• Protein Family: Hydroperoxy fatty acid reductase
• UniProt Gene Name: GPX1
• UniProt Synonym Gene Names: GPx-1; GSHPx-1
• UniProt Entry Name: GPX1_HUMAN

NCBI Description

This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GPX1: Protects the hemoglobin in erythrocytes from oxidative breakdown. Homotetramer. Interacts with MIEN1. Belongs to the glutathione peroxidase family.

Protein type: Other Amino Acids Metabolism - glutathione; Lipid Metabolism - arachidonic acid; Oxidoreductase; EC 1.11.1.9

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; cytosol; nucleus

Molecular Function: selenium binding; glutathione binding; phospholipid-hydroperoxide glutathione peroxidase activity; glutathione peroxidase activity; SH3 domain binding

Biological Process: response to nicotine; fat cell differentiation; blood vessel endothelial cell migration; myoblast proliferation; response to toxin; skeletal muscle fiber development; thermoregulation; negative regulation of caspase activity; vasodilation; regulation of gene expression, epigenetic; endothelial cell development; response to estradiol stimulus; regulation of mammary gland epithelial cell proliferation; response to selenium ion; sensory perception of sound; glutathione metabolic process; lipoxygenase pathway; UV protection; response to symbiotic bacterium; hydrogen peroxide catabolic process; response to folic acid; regulation of neuron apoptosis; response to glucose stimulus; response to gamma radiation; arachidonic acid metabolic process; negative regulation of inflammatory response to antigenic stimulus; aging; protein amino acid oxidation; nucleobase, nucleoside and nucleotide metabolic process; skeletal muscle regeneration; angiogenesis involved in wound healing; purine base metabolic process; response to xenobiotic stimulus; positive regulation of protein kinase B signaling cascade; response to reactive oxygen species; triacylglycerol metabolic process; response to hydrogen peroxide; response to lipid hydroperoxide; heart contraction; cell redox homeostasis; purine nucleotide catabolic process; interaction with symbiont; induction of apoptosis by oxidative stress

Disease: Glutathione Peroxidase Deficiency