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Western Blot (WB) (Western blot analysis of extracts from A549 cells, using POLR1C antibody.)

Rabbit anti-Human, Mouse POLR1C Polyclonal Antibody | anti-POLR1C antibody

POLR1C Antibody

Gene Names
POLR1C; AC40; RPA5; TCS3; HLD11; RPA39; RPA40; RPAC1; RPC40
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
POLR1C; Polyclonal Antibody; POLR1C Antibody; 40kDa; AA409007; AA959927; Ac2-127; AC40; AL024089; DNA directed RNA polymerase I subunit C; DNA directed RNA polymerases I and III 40 kDa polypeptide; DNA directed RNA polymerases I and III subunit RPAC1; DNA-directed RNA polymerase I subunit C; DNA-directed RNA polymerases I and III 40 kDa polypeptide; DNA-directed RNA polymerases I and III subunit RPAC1; MGC105583; MGC161175; POLR1E; Polymerase (RNA) I polypeptide C; 30kDa; RNA polymerase 1 1; RNA polymerases I and III subunit AC1; RP3-337H4.4; RPA39; RPA40; RPA5; RPAC1; RPAC1_HUMAN; RPC40; Rpo1 1; TCS3; anti-POLR1C antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
POLR1C antibody detects endogenous levels of total POLR1C
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
342
Applicable Applications for anti-POLR1C antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Nucleus.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (91%), Bovine (91%), Horse (100%), Sheep (91%), Rabbit (91%), Dog (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from A549 cells, using POLR1C antibody.)

Western Blot (WB) (Western blot analysis of extracts from A549 cells, using POLR1C antibody.)

Immunofluorescene (IF)

(MBS9605464 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Immunofluorescene (IF) (MBS9605464 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-POLR1C antibody
Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).
Subunit Structure: Component of the RNA polymerase I (Pol I) and RNA polymerase III (Pol III) complexes consisting of at least 13 and 17 subunits, respectively.
Similarity: Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 39 kD
Predicted: 40 kDa
NCBI Official Full Name
DNA-directed RNA polymerases I and III subunit RPAC1 isoform 2
NCBI Official Synonym Full Names
RNA polymerase I and III subunit C
NCBI Official Symbol
POLR1C
NCBI Official Synonym Symbols
AC40; RPA5; TCS3; HLD11; RPA39; RPA40; RPAC1; RPC40
NCBI Protein Information
DNA-directed RNA polymerases I and III subunit RPAC1
UniProt Protein Name
DNA-directed RNA polymerases I and III subunit RPAC1
UniProt Gene Name
POLR1C
UniProt Synonym Gene Names
POLR1E; DNA-directed RNA polymerase I subunit C; RNA polymerases I and III subunit AC1; RPA40

NCBI Description

The protein encoded by this gene is a subunit of both RNA polymerase I and RNA polymerase III complexes. The encoded protein is part of the Pol core element. Mutations in this gene have been associated with Treacher Collins syndrome (TCS) and hypomyelinating leukodystrophy 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft ().

Research Articles on POLR1C

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Product Notes

The POLR1C polr1c (Catalog #AAA9605464) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POLR1C Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's POLR1C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the POLR1C polr1c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLR1C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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