Rabbit anti-Human POLL Polyclonal Antibody | anti-POLL antibody

POLL, CT (POLL, DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa) (FITC)

Cat. Num. AAA6335634

• Gene Names: POLL; BETAN; POLKAPPA
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: POLL; Polyclonal Antibody; CT (POLL; DNA polymerase lambda; DNA polymerase beta-2; DNA polymerase kappa) (FITC); DNA polymerase kappa; anti-POLL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human POLL
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-POLL antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: POLL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 546~575 amino acids from the C-terminal region of human POLL.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of POLL Antibody (C-term) in HepG2 cell line lysates (35ug/lane). POLL (arrow) was detected using the purified Pab.)

Related Product Information for anti-POLL antibody

This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes.

Product Categories/Family for anti-POLL antibody

Antibodies; Enzymes; Polymerase

References

Brown, J.A., et al. J. Mol. Biol. 395(2):282-290(2010). van Loon, B., et al. Proc. Natl. Acad. Sci. U.S.A. 106(43):18201-18206(2009). Garcia-Diaz, M., et al. Nat. Struct. Mol. Biol. 16(9):967-972(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 291463287
• NCBI GeneID: 27343
• NCBI Accession #: NP_001167555.1
• NCBI GenBank Nucleotide #: NM_001174084.1
• UniProt Accession #: Q9UGP5; Q5JQP5; Q6NUM2; Q9HA10; Q9HB35; D3DR76
• Molecular Weight: 63,482 Da
• NCBI Official Full Name: DNA polymerase lambda isoform a
• NCBI Official Synonym Full Names: polymerase (DNA directed), lambda
• NCBI Official Symbol: POLL
• NCBI Official Synonym Symbols: BETAN; POLKAPPA
• NCBI Protein Information: DNA polymerase lambda; DNA polymerase kappa; DNA polymerase beta-2; DNA polymerase beta-N
• UniProt Protein Name: DNA polymerase lambda
• Protein Family: Pollen allergen
• UniProt Gene Name: POLL
• UniProt Synonym Gene Names: Pol Lambda; Pol beta2
• UniProt Entry Name: DPOLL_HUMAN

NCBI Description

This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010]

Uniprot Description

POLL: Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. Belongs to the DNA polymerase type-X family.

Protein type: DNA repair, damage; EC 2.7.7.7; Lyase; Transferase

Chromosomal Location of Human Ortholog: 10q23

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding; DNA-directed DNA polymerase activity; lyase activity

Biological Process: nucleotide-excision repair; somatic hypermutation of immunoglobulin genes; DNA replication