CD34 Polyclonal Antibody | anti-CD34 antibody

CD34 Antibody

Cat. Num. AAA857333

• Reactivity: Mouse, Human, Rat
• Applications: Western Blot, Immunocytochemistry
• Purity: Affinity purified
• Synonyms: CD34; Polyclonal Antibody; CD34 Antibody; anti-CD34 antibody

• Reactivity: Mouse, Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 1*TBS (pH7.4), 0.5%BSA, 25% Glycerol.
• Sequence Length: 385

• Applicable Applications for anti-CD34 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB: 1:5,000; ICC: 1:250
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human GSK3beta around the phosphorylation site of serine 9 (T-T-SP-F-A).
• Preservative: 0.05% Sodium Azide.
• Cellular Localization: Cell Membrane
• Positive Control: Mouse Brain
• Preparation and Storage: Store at +4 degree C after thawing. Aliquot store at-20 degree C or-80 degree C. Avoid repeated freeze/thaw cycles.

Testing Data

(Fig: Western blot analysis on mouse brain using anti-CD34 polyclonal antibody)

Related Product Information for anti-CD34 antibody

CD34 is a heavily glycosylated type I transmembrane molecule, that can be phoshorylated by a variety of kinases including Protein kinase C and Tyrosine kinases. CD34 antigen is expressed on small vessel endothelial cells and tumors of epithelial origin. CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. So CD34 is considered to be ideal marker for identifying and quantifying hematopoietic progenitor stem cells. With interaction with CrkL, CD34 becomes a substrate for PKC and CD34 surface expression is associated with activation of PKC.

References

1. Freund D., Wiebe G.J., Ehninger G., Corbeil D.; "Sequence analysis of the human CD34 antigen."; Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases. 2. The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004). 3. Sutherland D.R., Watt S.M., Dowden G., Karhi K., Baker M.A., Greaves M.F., Smart J.E.; "Structural and partial amino acid sequence analysis of the human hemopoietic progenitor cell antigen CD34."; Leukemia 2:793-803(1988).

NCBI and Uniprot Product Information

• NCBI GI #: 68342038
• NCBI GeneID: 947
• NCBI Accession #: NP_001020280.1
• NCBI GenBank Nucleotide #: NM_001025109.1
• UniProt Accession #: P28906; Q15970; Q15971; Q5JTA3; Q5JTA4; Q9UJB1; A8K664
• Molecular Weight: ~90/150kDa
• NCBI Official Full Name: hematopoietic progenitor cell antigen CD34 isoform a
• NCBI Official Synonym Full Names: CD34 molecule
• NCBI Official Symbol: CD34
• NCBI Protein Information: hematopoietic progenitor cell antigen CD34
• UniProt Protein Name: Hematopoietic progenitor cell antigen CD34
• Protein Family: Hematopoietic progenitor cell antigen
• UniProt Gene Name: CD34

NCBI Description

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.