Rabbit anti-Human PNPLA8 Polyclonal Antibody | anti-PNPLA8 antibody

PNPLA8, NT (PNPLA8, IPLA22, IPLA2G, Calcium-independent phospholipase A2-gamma, Intracellular membrane-associated calcium-independent phospholipase A2 gamma, PNPLA-gamma, Patatin-like phospholipase domain-containing protein 8, iPLA2-2) (FITC)

Cat. Num. AAA6335425

• Gene Names: PNPLA8; IPLA2G; IPLA2-2; iPLA2gamma; IPLA2(GAMMA)
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PNPLA8; Polyclonal Antibody; NT (PNPLA8; IPLA22; IPLA2G; Calcium-independent phospholipase A2-gamma; Intracellular membrane-associated calcium-independent phospholipase A2 gamma; PNPLA-gamma; Patatin-like phospholipase domain-containing protein 8; iPLA2-2) (FITC); iPLA2-2; anti-PNPLA8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PNPLA8 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PNPLA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219~248 amino acids from the N-terminal region of human PNPLA8.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PNPLA8 Antibody (N-term) western blot analysis in Hela,K562 cell line lysates (35ug/lane).This demonstrates the PNPLA8 antibody detected the PNPLA8 protein (arrow).)

Flow Cytometry (FC/FACS)

(PNPLA8 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PNPLA8 antibody

PNPLA8 catalyzes cleavage of fatty acids from phospholipids, thereby regulating membrane physical properties and the release of lipid second messengers and growth factors. Phospholipase A2 activity also modulates cellular growth programs, inflammation, and ion channel function.

Product Categories/Family for anti-PNPLA8 antibody

Antibodies; Enzymes; Phospholipase

References

Harper, M.T., et al. J. Thromb. Haemost. 6(10):1819-1821(2008). Wilson, P.A., et al. J. Lipid Res. 47(9):1940-1949(2006). Murakami, M., et al. J. Biol. Chem. 280(14):14028-14041(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 365192573
• NCBI GeneID: 50640
• NCBI Accession #: NP_001242936.1
• NCBI GenBank Nucleotide #: NM_001256007.1
• UniProt Accession #: Q9NP80; O95035; Q8N3I3; Q9H7T5; Q9NR17; Q9NUN2; Q9NZ79; A4D0S1; C9JZI4
• Molecular Weight: 88,477 Da
• NCBI Official Full Name: calcium-independent phospholipase A2-gamma isoform 1
• NCBI Official Synonym Full Names: patatin-like phospholipase domain containing 8
• NCBI Official Symbol: PNPLA8
• NCBI Official Synonym Symbols: IPLA2G; IPLA2-2; iPLA2gamma; IPLA2(GAMMA)
• NCBI Protein Information: calcium-independent phospholipase A2-gamma; PNPLA-gamma; iPLA2 gamma; iPLA2-gamma; patatin-like phospholipase domain-containing protein 8; membrane-associated calcium-independent phospholipase A2 gamma; intracellular membrane-associated calcium-independen
• UniProt Protein Name: Calcium-independent phospholipase A2-gamma
• Protein Family: Calcium-independent phospholipase
• UniProt Gene Name: PNPLA8
• UniProt Synonym Gene Names: IPLA22; IPLA2G; iPLA2-gamma
• UniProt Entry Name: PLPL8_HUMAN

NCBI Description

This gene encodes a member of the patatin-like phospholipase domain containing protein family. Members of this family are phospholipases which catalyze the cleavage of fatty acids from membrane phospholipids. The product of this gene is a calcium-independent phospholipase. A pseudogene of this gene is located on the X chromosome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

PNPLA8: Calcium-independent phospholipase A2, which catalyzes the hydrolysis of the sn-2 position of glycerophospholipids, PtdSer and to a lower extent PtdCho. Cleaves membrane phospholipids. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Phospholipase; Membrane protein, integral; EC 3.1.1.5

Chromosomal Location of Human Ortholog: 7q31

Cellular Component: Golgi membrane; peroxisomal membrane; endoplasmic reticulum membrane; membrane; perinuclear region of cytoplasm; integral to membrane; peroxisome; intracellular

Molecular Function: calcium-independent phospholipase A2 activity; ATP binding; lysophospholipase activity

Biological Process: cell death; phosphatidylethanolamine catabolic process; phospholipid metabolic process; glycerophospholipid biosynthetic process; arachidonic acid metabolic process; linoleic acid metabolic process; fatty acid metabolic process; prostaglandin biosynthetic process; arachidonic acid secretion

Disease: Mitochondrial Myopathy With Lactic Acidosis