Goat PNPLA3/Adiponutrin Polyclonal Antibody | anti-PNPLA3 antibody

Goat anti-PNPLA3/Adiponutrin Antibody

Cat. Num. AAA421616

• Gene Names: Pnpla3; Adpn
• Reactivity: Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: PNPLA3/Adiponutrin; Polyclonal Antibody; Goat anti-PNPLA3/Adiponutrin Antibody; Pnpla3; patatin-like phospholipase domain containing 3; Adpn; adiponutrin; Pnpla3 antibody; patatin-like phospholipase domain containing 3 antibody; Adpn antibody; adiponutrin antibody; Adiponutrin; PNPLA3 / Adiponutrin; anti-PNPLA3 antibody

• Host: Goat
• Reactivity: Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: EHDICPKVKSTN
• Sequence Length: 384

• Applicable Applications for anti-PNPLA3 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Immunohistochemistry: In paraffin embedded Human Skin shows membranous staining of basak kayer cells. Recommended concentration, 4-6ug/ml.; Western Blot: Approx 45kDa band observed in Mouse Heart lysates and in Mouse and Rat Adrenal gland lysates (calculated MW of 42.6kDa according to Mouse NP_473429.2 and 47.1kDa according to Rat NP_)001269253.1. Recommended concentration: 0.01-0.03ug/ml.
• Immunogen: Peptide with sequence EHDICPKVKSTN, from the internal region of the protein sequence according to NP_473429.2.
• Epitope: Internal region
• Note: This antibody is raised against a peptide derived from the Mouse protein sequence and this peptide differs from Human only in position 2.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0. 05ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

((0. 01ug/ml) staining of Mouse (A) and Rat (B) Adrenal gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

((3.8ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 144226245
• NCBI GeneID: 116939
• NCBI Accession #: NP_473429.2
• NCBI GenBank Nucleotide #: NM_054088.3
• Molecular Weight: 45,772 Da
• NCBI Official Full Name: patatin-like phospholipase domain-containing protein 3
• NCBI Official Synonym Full Names: patatin-like phospholipase domain containing 3
• NCBI Official Symbol: Pnpla3
• NCBI Official Synonym Symbols: Adpn
• NCBI Protein Information: patatin-like phospholipase domain-containing protein 3
• UniProt Protein Name: Patatin-like phospholipase domain-containing protein 3
• Protein Family: Patatin-like phospholipase domain-containing protein
• UniProt Gene Name: Pnpla3
• UniProt Synonym Gene Names: Adpn; iPLA2-epsilon
• UniProt Entry Name: PLPL3_MOUSE

Uniprot Description

PNPLA3: Multifunctional enzyme which has both triacylglycerol lipase and acylglycerol O-acyltransferase activities. Genetic variations in PNPLA3 are a cause of susceptibility to non-alcoholic fatty liver disease type 1 (NAFLD1). A condition characterized by an accumulation of excess triglyceride in the liver, a condition known as hepatic steatosis (or fatty liver), which is associated with adverse metabolic consequences, including insulin resistance and dyslipidemia. In a subset of individuals, hepatic steatosis promotes an inflammatory response in the liver, referred to as steatohepatitis, which can progress to cirrhosis and liver cancer. NAFLD is the most common form of liver disease in Western countries. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerolipid; Amino Acid Metabolism - tyrosine; Transferase; Membrane protein, integral; Amino Acid Metabolism - phenylalanine; EC 3.1.1.3; Lipid Metabolism - glycerophospholipid; Secondary Metabolites Metabolism - limonene and pinene degradation; Phospholipase

Cellular Component: integral to membrane; membrane

Molecular Function: 3-hydroxybutyryl-CoA thiolase activity; 3-ketopimelyl-CoA thiolase activity; acetyltransferase activity; acyl-CoA N-acyltransferase activity; acylglycerol O-acyltransferase activity; azetidine-2-carboxylic acid acetyltransferase activity; benzoyl acetate-CoA thiolase activity; C-acyltransferase activity; C-palmitoyltransferase activity; carnitine O-acyltransferase activity; dihydrolipoamide branched chain acyltransferase activity; dihydrolipoamide S-acyltransferase activity; diolein transacylation activity; glucosaminyl-phosphotidylinositol O-acyltransferase activity; hydrolase activity; keto acid formate lyase activity; L-2-aminoadipate N-acetyltransferase activity; malonyltransferase activity; mono-olein transacylation activity; myristoyltransferase activity; N-acetyltransferase activity; N-acyltransferase activity; N-palmitoyltransferase activity; N-succinyltransferase activity; O-acetyltransferase activity; O-acyltransferase activity; O-octanoyltransferase activity; O-palmitoyltransferase activity; O-sinapoyltransferase activity; O-succinyltransferase activity; octanoyltransferase activity; palmitoleoyl [acyl-carrier-protein]-dependent acyltransferase activity; palmitoyltransferase activity; peptidyl-lysine N6-myristoyltransferase activity; peptidyl-lysine N6-palmitoyltransferase activity; phospholipase A2 activity; protein-cysteine S-acyltransferase activity; protein-cysteine S-myristoyltransferase activity; Ras palmitoyltransferase activity; S-acetyltransferase activity; S-acyltransferase activity; S-malonyltransferase activity; S-succinyltransferase activity; serine O-acyltransferase activity; sinapoyltransferase activity; sterol O-acyltransferase activity; succinyltransferase activity; transferase activity; transferase activity, transferring acyl groups; triacylglycerol lipase activity

Biological Process: lipid catabolic process; lipid metabolic process; metabolic process; triacylglycerol biosynthetic process; triacylglycerol catabolic process