Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PNKP antibody.)
Rabbit anti-Human PNKP Polyclonal Antibody | anti-PNKP antibody
PNKP Polyclonal Antibody
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PNKP antibody.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PNKP antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cell using PNKP antibody. Green?GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.)
Immunofluorescence (IF)
(Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cell using PNKP antibody. Green?GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.)
NCBI and Uniprot Product Information
NCBI Description
This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.[provided by RefSeq, Sep 2010]
Uniprot Description
Pnk1: polynucleotide kinase 3'-phosphatase. May be involved in double-strand break repair by non-homologous end joining. Required for normal response to DNA damage by gamma-radiation or camptothecin in S. pombe.
Protein type: EC 3.1.3.32; Kinase, other; EC 2.7.1.78; Phosphatase (non-protein); DNA-binding
Chromosomal Location of Human Ortholog: 19q13.3-q13.4
Cellular Component: membrane; nucleolus; nucleoplasm; nucleus
Molecular Function: ATP binding; ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; damaged DNA binding; double-stranded DNA binding; endonuclease activity; nucleotide kinase activity; polynucleotide 3'-phosphatase activity; protein binding; purine nucleotide binding
Biological Process: base-excision repair; dephosphorylation; DNA damage response, detection of DNA damage; DNA repair; DNA-dependent DNA replication; nucleotide phosphorylation; nucleotide-excision repair, DNA damage removal; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; response to oxidative stress; response to radiation
Disease: Ataxia-oculomotor Apraxia 4; Microcephaly, Seizures, And Developmental Delay
Research Articles on PNKP
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Product Notes
The PNKP pnkp (Catalog #AAA2538149) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PNKP Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PNKP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000, IHC: 1:50-1:200. Researchers should empirically determine the suitability of the PNKP pnkp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PNKP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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