Rabbit anti-Human PNKP Polyclonal Antibody | anti-PNKP antibody

PNKP, NT (PNKP, Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase, Polynucleotide 3'-phosphatase, 2'(3')-polynucleotidase, Polynucleotide 5'-hydroxyl-kinase) (Biotin)

Cat. Num. AAA6335270

• Gene Names: PNKP; PNK; MCSZ; EIEE10
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PNKP; Polyclonal Antibody; NT (PNKP; Bifunctional polynucleotide phosphatase/kinase; DNA 5'-kinase/3'-phosphatase; Polynucleotide kinase-3'-phosphatase; Polynucleotide 3'-phosphatase; 2'(3')-polynucleotidase; Polynucleotide 5'-hydroxyl-kinase) (Biotin); Polynucleotide 5'-hydroxyl-kinase; anti-PNKP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PNKP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PNKP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-138 amino acids from the N-terminal region of human PNKP.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PNKP Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PNKP antibody detected the PNKP protein (arrow).)

Western Blot (WB)

(Western blot analysis of PNKP (arrow) using rabbit polyclonal PNKP Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PNKP gene.)

Related Product Information for anti-PNKP antibody

This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.

Product Categories/Family for anti-PNKP antibody

Antibodies; Protein Kinases

References

Arora, M., et al. Leukemia 24(8):1470-1475(2010). Thyagarajan, B., et al. Biol. Blood Marrow Transplant. 16(8):1084-1089(2010). Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010). Shen, J., et al. Nat. Genet. 42(3):245-249(2010). Ali, A.A., et al. Nucleic Acids Res. 37(5):1701-1712(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 31543419
• NCBI GeneID: 11284
• NCBI Accession #: NP_009185.2
• NCBI GenBank Nucleotide #: NM_007254.3
• UniProt Accession #: Q96T60; Q9BUL2; Q9P1V2; Q9UKU8; Q9UNF8; Q9UNI0
• Molecular Weight: 53,004 Da
• NCBI Official Full Name: bifunctional polynucleotide phosphatase/kinase
• NCBI Official Synonym Full Names: polynucleotide kinase 3'-phosphatase
• NCBI Official Symbol: PNKP
• NCBI Official Synonym Symbols: PNK; MCSZ; EIEE10
• NCBI Protein Information: bifunctional polynucleotide phosphatase/kinase; DNA 5'-kinase/3'-phosphatase; Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP)
• UniProt Protein Name: Bifunctional polynucleotide phosphatase/kinase
• UniProt Gene Name: PNKP
• UniProt Entry Name: PNKP_HUMAN

NCBI Description

This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.[provided by RefSeq, Sep 2010]

Uniprot Description

Pnk1: polynucleotide kinase 3'-phosphatase. May be involved in double-strand break repair by non-homologous end joining. Required for normal response to DNA damage by gamma-radiation or camptothecin in S. pombe.

Protein type: Kinase, other; EC 3.1.3.32; EC 2.7.1.78; DNA-binding; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: nucleoplasm; membrane; nucleolus; nucleus

Molecular Function: protein binding; ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; polynucleotide 3'-phosphatase activity; purine nucleotide binding; endonuclease activity; double-stranded DNA binding; damaged DNA binding; nucleotide kinase activity; ATP binding

Biological Process: response to radiation; dephosphorylation; DNA damage response, detection of DNA damage; nucleotide-excision repair, DNA damage removal; response to oxidative stress; DNA repair; DNA-dependent DNA replication; nucleotide phosphorylation

Disease: Microcephaly, Seizures, And Developmental Delay; Ataxia-oculomotor Apraxia 4