Rabbit PMRD Polyclonal Antibody | anti-PMRD antibody

Rabbit anti-Escherichia coli (strain K12) PMRD Polyclonal Antibody

• Gene Names: pmrD; ECK2253; JW2254
• Reactivity: E Coli (strain K12)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: PMRD; Polyclonal Antibody; Rabbit anti-Escherichia coli (strain K12) PMRD Polyclonal Antibody; PMRD Antibody; Signal transduction protein PmrD; BasR post-transcriptional activator; Polymyxin resistance protein PmrD; pmrD b2259 JW2254; anti-PMRD antibody

• Host: Rabbit
• Reactivity: E Coli (strain K12)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-PMRD antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Escherichia coli (strain K12) pmrD protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 90111410
• NCBI GeneID: 945130
• NCBI Accession #: NP_416762.2
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P37590; P76475; P77155; Q5MN33; Q5MN36; Q5MN40; Q5MN44; Q5MN45; Q5MN54; Q5MN59; Q5MN60; Q5MN62
• Molecular Weight: 9,871 Da
• NCBI Official Full Name: inactive two-component system connector protein
• NCBI Official Symbol: pmrD
• NCBI Official Synonym Symbols: ECK2253; JW2254
• NCBI Protein Information: inactive two-component system connector protein
• UniProt Protein Name: Signal transduction protein PmrD
• Protein Family: Signal transduction protein
• UniProt Gene Name: pmrD

NCBI Description

In Salmonella but not Escherichia coli, low Mg(2+) PhoP-activated PmrD controls PmrA(BasR)/PmrB(BasS) activity post-translationally by binding to phospho-PmrA(BasR) and inhibiting its dephosphorylation but not its phosphotransfer, connecting the PhoPQ and PmrAB two-component systems and allowing for an amplified and persistent PmrAB response (Kato, 2007). [More information is available at EcoGene: EG12671]. PmrD is similar to the Salmonella enterica serovar Typhimurium PmrD, which confers resistance to polymyxin B when it is overexpressed ; in Salmonella, PmrD controls the activity of the PmrA-PmrB two-component system at a post-transcriptional level . [More information is available at EcoCyc: G7172].

Uniprot Description

Interacts with phosphorylated BasR protein to mediate transcriptional induction of BasR-activated genes to induce polymyxin resistance in some natural isolates.