Rabbit anti-Mouse PMAIP1 Polyclonal Antibody | anti-PMAIP1 antibody

PMAIP1 (Phorbol-12-Myristate-13-Acetate Induced Protein 1, APR, NOXA)

Cat. Num. AAA613673

• Gene Names: PMAIP1; APR; NOXA
• Reactivity: Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified; IgG fractionation
• Synonyms: PMAIP1; Polyclonal Antibody; PMAIP1 (Phorbol-12-Myristate-13-Acetate Induced Protein 1; APR; NOXA); Anti -PMAIP1 (Phorbol-12-Myristate-13-Acetate Induced Protein 1; anti-PMAIP1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: Recognizes Mouse Phorbol-12-myristate-13-acetate-induced Protein 1 (PMAIP1). Species Crossreactivity: Human, Mouse, Rat.
• Purity/Purification: Purified; IgG fractionation
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-PMAIP1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blotting, Immunohistochemistry.; Dilution: Western Blotting: 0.5-1ug/ml; Immunohistochemistry: 5-10ug/ml
• Immunogen: Synthetic peptide, KLH-conjugated. Epitope: synthetic peptide corresponding to 17 amino acids at the amino terminus of mouse Noxa
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(PMAIP1 / NOXA (5ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human pancreas, followed by biotinylated goat rabbit IgG secondary, alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-PMAIP1 antibody

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes including p53R2, p53AIP1, and PUMA. A new p53 target gene, Noxa, was recently identified (1), which encodes a protein belonging to the subfamily of BH3-only proapoptic proteins. Noxa and PUMA are both transcriptional targets of p53 and BH3-only proteins. X-ray irradiation increased p53-dependent Noxa mRNA and protein levels. Noxa, when ectopically expressed, interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9 (1). Noxa, like PUMA, localized to mitochondria and induces apoptosis in response to p53 (1-3). Noxa and PUMA may represent direct mediators of p53-induced apoptosis. Increased levels of p53 and its target gene Noxa was found in the impaired tumor development (4).

Product Categories/Family for anti-PMAIP1 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 10863923
• NCBI GeneID: 5366
• NCBI Accession #: NP_066950.1
• NCBI GenBank Nucleotide #: NM_021127.2
• UniProt Accession #: Q13794; B2R4T7; Q8N589
• Molecular Weight: 6,030 Da
• NCBI Official Full Name: phorbol-12-myristate-13-acetate-induced protein 1
• NCBI Official Synonym Full Names: phorbol-12-myristate-13-acetate-induced protein 1
• NCBI Official Symbol: PMAIP1
• NCBI Official Synonym Symbols: APR; NOXA
• NCBI Protein Information: phorbol-12-myristate-13-acetate-induced protein 1; protein Noxa; OTTHUMP00000163652; PMA-induced protein 1; immediate-early-response protein APR; adult T cell leukemia-derived PMA-responsive
• UniProt Protein Name: Phorbol-12-myristate-13-acetate-induced protein 1
• Protein Family: Phorbol-12-myristate-13-acetate-induced protein
• UniProt Gene Name: PMAIP1
• UniProt Synonym Gene Names: NOXA
• UniProt Entry Name: APR_HUMAN

Uniprot Description

NOXA: Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1. Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1. Interacts with MCL1, BCL2A1 and BAX. Up-regulated by p53/TP53, phorbol esters, double- stranded RNA, IFNB1/IFN-beta and viruses. Highly expressed in adult T-cell leukemia cell line. Belongs to the PMAIP1 family.

Protein type: Mitochondrial; Apoptosis

Chromosomal Location of Human Ortholog: 18q21.32

Cellular Component: mitochondrial outer membrane; mitochondrion; nucleus; cytosol

Molecular Function: protein binding

Biological Process: proteasomal protein catabolic process; caspase activation; release of cytochrome c from mitochondria; apoptosis; positive regulation of apoptosis; response to dsRNA; positive regulation of caspase activity; positive regulation of protein oligomerization; cellular response to glucose starvation; negative regulation of fibroblast proliferation; positive regulation of neuron apoptosis; T cell homeostasis; positive regulation of DNA damage response, signal transduction by p53 class mediator; regulation of mitochondrial membrane permeability; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; defense response to virus; response to X-ray; response to UV