Rabbit anti-Human PLOD1 Polyclonal Antibody | anti-PLOD1 antibody

PLOD1, NT (PLOD1, LLH, PLOD, Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1, Lysyl hydroxylase 1) (APC)

Cat. Num. AAA6335049

• Gene Names: PLOD1; LH; LH1; LLH; EDS6; PLOD
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PLOD1; Polyclonal Antibody; NT (PLOD1; LLH; PLOD; Procollagen-lysine; 2-oxoglutarate 5-dioxygenase 1; Lysyl hydroxylase 1) (APC); Lysyl hydroxylase 1; anti-PLOD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PLOD1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PLOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-102 amino acids from the N-terminal region of human PLOD1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PLOD1 Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the PLOD1 antibody detected the PLOD1 protein (arrow).)

Immunohistochemistry (IHC)

(PLOD1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLOD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PLOD1 Antibody (N-term) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PLOD1 antibody

Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity.

Product Categories/Family for anti-PLOD1 antibody

Antibodies; Enzymes; Oxidase

References

Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010): Huang, Q.Y., et al. Bone 44(5):984-988(2009). Yamada, Y., et al. Int. J. Mol. Med. 19(5):791-801(2007). Tasker, P.N., et al. Osteoporos Int 17(7):1078-1085(2006). Giunta, C., et al. Mol. Genet. Metab. 86 (1-2), 269-276 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 32307144
• NCBI GeneID: 5351
• NCBI Accession #: NP_000293.2
• NCBI GenBank Nucleotide #: NM_000302.3
• UniProt Accession #: Q02809; Q96AV9; Q9H132; B4DR87
• Molecular Weight: 88,273 Da
• NCBI Official Full Name: procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 isoform 2
• NCBI Official Synonym Full Names: procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
• NCBI Official Symbol: PLOD1
• NCBI Official Synonym Symbols: LH; LH1; LLH; EDS6; PLOD
• NCBI Protein Information: procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
• UniProt Protein Name: Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
• Protein Family: Procollagen-lysine,2-oxoglutarate 5-dioxygenase
• UniProt Gene Name: PLOD1
• UniProt Synonym Gene Names: LLH; PLOD; LH1

NCBI Description

Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

Uniprot Description

Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.