Immunofluorescence (IF)
(MBS9605926 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human PLK5 Polyclonal Antibody | anti-PLK5 antibody
PLK5 Antibody
Gene Names
PLK5; PLK-5; PLK5P; SgK384ps
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PLK5; Polyclonal Antibody; PLK5 Antibody; FG060302; Inactive serine/threonine-protein kinase PLK5; PLK-5; PLK5P; polo like kinase 5; pseudogene; Polo-like kinase 5; SgK384ps; anti-PLK5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
PLK5 antibody detects endogenous levels of total PLK5
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
336
Applicable Applications for anti-PLK5 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Nucleus
Tissue Specificity
Expressed in the brain, neurons and glial cells. Also expressed in highly differentiated cells, such as the serous acini in the parotid gland, distal and proximal tubules of the kidney, tubules of the seminal gland, Kupffer cells and some hepatocytes in the liver, and some cells in the germinal center of lymph nodes (at protein level).
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9605926 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9605926 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from COLO cells using PLK5 antibody.The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from COLO cells using PLK5 antibody.The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-PLK5 antibody
Function: Inactive serine/threonine-protein kinase that plays a role in cell cycle progression and neuronal differentiation.
Similarity: The truncated protein kinase domain is predicted to be catalytically inactive; has lost the main activatory autophosphorylation site and the conserved key residues involved in phospho-substrate. The C-terminal region (containing the POLO box domain) is sufficient for inducing cell cycle arrest. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Similarity: The truncated protein kinase domain is predicted to be catalytically inactive; has lost the main activatory autophosphorylation site and the conserved key residues involved in phospho-substrate. The C-terminal region (containing the POLO box domain) is sufficient for inducing cell cycle arrest. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 55 kD
Predicted: 37 kDa
Predicted: 37 kDa
NCBI Official Full Name
inactive serine/threonine-protein kinase PLK5
NCBI Official Synonym Full Names
polo like kinase 5
NCBI Official Symbol
PLK5
NCBI Official Synonym Symbols
PLK-5; PLK5P; SgK384ps
NCBI Protein Information
inactive serine/threonine-protein kinase PLK5
UniProt Protein Name
Inactive serine/threonine-protein kinase PLK5
Protein Family
UniProt Gene Name
PLK5
UniProt Synonym Gene Names
PLK5P; PLK-5
Uniprot Description
Inactive serine/threonine-protein kinase that plays a role in cell cycle progression and neuronal differentiation.
Research Articles on PLK5
Similar Products
Product Notes
The PLK5 plk5 (Catalog #AAA9605926) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PLK5 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLK5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the PLK5 plk5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLK5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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