Typical Testing Data/Standard Curve (for reference only)
Human Serine/threonine-protein kinase PLK1 ELISA Kit | PLK1 elisa kit
Human Serine/threonine-protein kinase PLK1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine/threonine-protein kinase PLK1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forSerine/threonine-protein kinase PLK1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Serine/threonine-protein kinase PLK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Serine/threonine-protein kinase PLK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]
Uniprot Description
PLK1: a kinase of the PLK family. Contains a polo-box domain (PBD), a specific phosphoserine or phosphothreonine binding domain. Substrates include BRCA2, Myt1, NudC, Cdc25C, cyclin B1, Nlp and other mitotic proteins. Inhibited by ATR. Plays a role in regulation of cytokinesis and coordinating M-phase events.
Protein type: Kinase, protein; Protein kinase, Other; EC 2.7.11.21; Protein kinase, Ser/Thr (non-receptor); Other group; PLK family
Chromosomal Location of Human Ortholog: 16p12.2
Cellular Component: centrosome; chromatin; cytoplasm; cytosol; kinetochore; microtubule cytoskeleton; midbody; nucleolus; nucleoplasm; nucleus; spindle; spindle microtubule; spindle midzone; spindle pole; synaptonemal complex
Molecular Function: ATP binding; kinase activity; microtubule binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity
Biological Process: anaphase-promoting complex activation during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell proliferation; centrosome organization and biogenesis; cytokinesis; cytokinesis after mitosis; establishment of protein localization; female meiosis chromosome segregation; G2/M transition DNA damage checkpoint; G2/M transition of mitotic cell cycle; homologous chromosome segregation; microtubule bundle formation; mitosis; mitotic cell cycle; mitotic cell cycle spindle assembly checkpoint; mitotic metaphase/anaphase transition; mitotic nuclear envelope disassembly; mitotic sister chromatid segregation; negative regulation of apoptosis; negative regulation of cyclin-dependent protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; organelle organization and biogenesis; peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of proteolysis; positive regulation of ubiquitin-protein ligase activity; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein amino acid phosphorylation; protein destabilization; protein ubiquitination; regulation of cell cycle; regulation of mitotic cell cycle; regulation of mitotic metaphase/anaphase transition; regulation of protein binding; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; small GTPase mediated signal transduction
Research Articles on PLK1
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Product Notes
The Human PLK1 plk1 (Catalog #AAA2885387) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2885387 ELISA Kit recognizes Human PLK1. It is sometimes possible for the material contained within the vial of "Serine/threonine-protein kinase PLK1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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