Rabbit anti-Human PLK1 Polyclonal Antibody | anti-PLK1 antibody

PLK1, CT (PLK1, PLK, Serine/threonine-protein kinase PLK1, Polo-like kinase 1, Serine/threonine-protein kinase 13) (MaxLight 490)

Cat. Num. AAA6334977

• Gene Names: PLK1; PLK; STPK13
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: PLK1; Polyclonal Antibody; CT (PLK1; PLK; Serine/threonine-protein kinase PLK1; Polo-like kinase 1; Serine/threonine-protein kinase 13) (MaxLight 490); Serine/threonine-protein kinase 13; anti-PLK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PLK1.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight490.

• Applicable Applications for anti-PLK1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 573-603 in the C-terminal region of human PLK1, UniProt Accession #P53350; Q07832. Species sequence homology: mouse
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis in A375 cell lysate usingMBS642497. PLK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS642497, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-PLK1 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Product Categories/Family for anti-PLK1 antibody

Antibodies; Protein Kinases

References

1. Ree, A.H., et al., Oncogene 22(55):8952-8955 (2003). 2. Cheng, K.Y., et al., EMBO J. 22(21):5757-5768 (2003). 3. Elia, A.E., et al., Cell 115(1):83-95 (2003). 4. Lin, H.R., et al., J. Biol. Chem. 278(38):35979-35987 (2003). 5. Neef, R., et al., J. Cell Biol. 162(5):863-875 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 21359873
• NCBI GeneID: 5347
• NCBI Accession #: NP_005021.2
• NCBI GenBank Nucleotide #: NM_005030.3
• UniProt Accession #: P53350; Q07832
• Molecular Weight: 68,255 Da
• NCBI Official Full Name: serine/threonine-protein kinase PLK1
• NCBI Official Synonym Full Names: polo-like kinase 1
• NCBI Official Symbol: PLK1
• NCBI Official Synonym Symbols: PLK; STPK13
• NCBI Protein Information: serine/threonine-protein kinase PLK1; PLK-1; polo like kinase; polo (Drosophia)-like kinase; serine/threonine-protein kinase 13; cell cycle regulated protein kinase
• UniProt Protein Name: Serine/threonine-protein kinase PLK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PLK1
• UniProt Synonym Gene Names: PLK; PLK-1; STPK13
• UniProt Entry Name: PLK1_HUMAN

NCBI Description

The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]

Uniprot Description

PLK1: a kinase of the PLK family. Contains a polo-box domain (PBD), a specific phosphoserine or phosphothreonine binding domain. Substrates include BRCA2, Myt1, NudC, Cdc25C, cyclin B1, Nlp and other mitotic proteins. Inhibited by ATR. Plays a role in regulation of cytokinesis and coordinating M-phase events.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.21; Protein kinase, Other; Kinase, protein; Other group; PLK family

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: spindle pole; centrosome; cytosol; nucleoplasm; kinetochore; microtubule cytoskeleton; spindle microtubule; cytoplasm; nucleolus; spindle; midbody; spindle midzone; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; microtubule binding; kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of cell cycle; positive regulation of proteolysis; regulation of mitotic cell cycle; mitotic nuclear envelope disassembly; cytokinesis; protein ubiquitination; anaphase-promoting complex activation during mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; response to antibiotic; establishment of protein localization; cytokinesis after mitosis; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; centrosome organization and biogenesis; positive regulation of ubiquitin-protein ligase activity; mitotic cell cycle spindle assembly checkpoint; G2/M transition of mitotic cell cycle; mitosis; mitotic sister chromatid segregation; protein destabilization; organelle organization and biogenesis; regulation of mitotic metaphase/anaphase transition; negative regulation of cyclin-dependent protein kinase activity; cell proliferation; peptidyl-serine phosphorylation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of protein binding; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; polar body extrusion after meiotic divisions; mitotic cell cycle; G2/M transition DNA damage checkpoint; microtubule bundle formation; mitotic metaphase/anaphase transition; negative regulation of apoptosis