Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PLEK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 10s.)
Rabbit anti-Human PLEK Polyclonal Antibody | anti-PLEK antibody
PLEK Polyclonal Antibody
Gene Names
PLEK; P47
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Synonyms
PLEK; Polyclonal Antibody; PLEK Polyclonal Antibody; P47; anti-PLEK antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence
YLQPAGDMSKSAVDGTAENPFLDNPDAFYYFPDSGFFCEENSSDDDVILKEEFRGVIIKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGAEDPLGAIHLRGCVVTSVESNSNGRKSEEENLFEIITADEVHYFLQAATPKERTEWIRAIQMASRTGK
Sequence Length
350
Applicable Applications for anti-PLEK antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IHC: 1:50 - 1:200
Immunogen
Recombinant protein of human PLEK
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PLEK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PLEK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 10s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate using PLEK antibody at dilution of 1:200 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate using PLEK antibody at dilution of 1:200 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human stomach using PLEK antibody at dilution of 1:200 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human stomach using PLEK antibody at dilution of 1:200 (40x lens).)
Product Categories/Family for anti-PLEK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 40kDa
Observed: 40kDa
Observed: 40kDa
NCBI Official Full Name
pleckstrin
NCBI Official Synonym Full Names
pleckstrin
NCBI Official Symbol
PLEK
NCBI Official Synonym Symbols
P47
NCBI Protein Information
pleckstrin
UniProt Protein Name
Pleckstrin
Protein Family
UniProt Gene Name
PLEK
UniProt Synonym Gene Names
P47; p47
Uniprot Description
Major protein kinase C substrate of platelets.
Research Articles on PLEK
Similar Products
Product Notes
The PLEK plek (Catalog #AAA9133381) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PLEK Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLEK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the PLEK plek for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: YLQPAGDMSK SAVDGTAENP FLDNPDAFYY FPDSGFFCEE NSSDDDVILK EEFRGVIIKQ GCLLKQGHRR KNWKVRKFIL REDPAYLHYY DPAGAEDPLG AIHLRGCVVT SVESNSNGRK SEEENLFEII TADEVHYFLQ AATPKERTEW IRAIQMASRT GK. It is sometimes possible for the material contained within the vial of "PLEK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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