Typical Testing Data/Standard Curve (for reference only)
Human Tyrosyl tRNA synthetase, cytoplasmic (YARS) ELISA Kit | YARS elisa kit
Human Tyrosyl tRNA synthetase, cytoplasmic (YARS) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: YARS ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-YARS antibody and an YARS-HRP conjugate. The assay sample and buffer are incubated together with YARS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the YARS concentration since YARS from samples and YARS-HRP conjugate compete for the anti-YARS antibody binding site. Since the number of sites is limited, as more sites are occupied by YARS from the sample, fewer sites are left to bind YARS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YARS concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq, Jul 2008]
Uniprot Description
YARS: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr). Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC). CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec. Belongs to the class-I aminoacyl-tRNA synthetase family.
Protein type: EC 6.1.1.1; Ligase
Chromosomal Location of Human Ortholog: 1p35.1
Cellular Component: extracellular space; cytoplasm; nucleus; cytosol
Molecular Function: signal transducer activity; tyrosine-tRNA ligase activity; ATP binding; isoleucine-tRNA ligase activity; tRNA binding; interleukin-8 receptor binding
Biological Process: isoleucyl-tRNA aminoacylation; tyrosyl-tRNA aminoacylation; tRNA aminoacylation for protein translation; apoptosis; gene expression; signal transduction
Disease: Charcot-marie-tooth Disease, Dominant Intermediate C
Research Articles on YARS
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Product Notes
The Human YARS yars (Catalog #AAA7215349) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7215349 ELISA Kit recognizes Human YARS. It is sometimes possible for the material contained within the vial of "Tyrosyl tRNA synthetase, cytoplasmic (YARS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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