Rabbit Plectin Polyclonal Antibody | anti-PLEC antibody

Plectin Polyclonal Antibody

Cat. Num. AAA3013274

• Gene Names: PLEC; HD1; PCN; EBS1; EBSO; PLTN; EBSMD; EBSND; EBSOG; EBSPA; PLEC1; LGMD2Q; PLEC1b; LGMDR17
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: Plectin; Polyclonal Antibody; Plectin Polyclonal Antibody; PCN; PLTN; HD1; PLEC; EBS1; EBSO; Hemidesmosomal protein 1; pleC; PLEC1; PLEC1b; Plectin 1; Plectin 1 intermediate filament binding protein 500kDa; Plectin 6; Plectin-1; anti-PLEC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Plectin polyclonal antibody detects endogenous levels of Plectin protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 4574

• Applicable Applications for anti-PLEC antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:1,000; IHC: 1:100-1:500
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Plectin antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Plectin antibody. Counter stained with hematoxylin.)

Related Product Information for anti-PLEC antibody

Plectin is an abundant cytoskeletal protein that is involved in cytoplasm stabilization. Plectin has been shown to crosslink intermediate filaments to microtubules and microfilaments, and to anchor intermediate filaments to the plasma and nuclear membranes. Plectin binds both Lamin B and vimentin, and this binding is regulated by a variety of protein kinases. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions. Plectin is also a substrate for p34cdc2 kinase. Several alternative splice isoforms of plectin are known to exist. Mutations in human plectin are known to cause epidermolysis bullosa simplex with muscular dystrophy (EBS-MD).

NCBI and Uniprot Product Information

• NCBI GI #: 47607492
• NCBI GeneID: 5339
• NCBI Accession #: NP_000436.2
• NCBI GenBank Nucleotide #: NP_000436.2
• UniProt Accession #: Q15149; Q9QXS1; P30427
• Molecular Weight: 531kDa
• NCBI Official Full Name: plectin isoform 1c
• NCBI Official Synonym Full Names: plectin
• NCBI Official Symbol: PLEC
• NCBI Official Synonym Symbols: HD1; PCN; EBS1; EBSO; PLTN; EBSMD; EBSND; EBSOG; EBSPA; PLEC1; LGMD2Q; PLEC1b; LGMDR17
• NCBI Protein Information: plectin
• UniProt Protein Name: Plectin
• Protein Family: Plectin
• UniProt Gene Name: PLEC
• UniProt Synonym Gene Names: PLEC1; PCN; PLTN; HD1
• UniProt Entry Name: PLEC_HUMAN

NCBI Description

Plectin is a prominent member of an important family of structurally and in part functionally related proteins, termed plakins or cytolinkers, that are capable of interlinking different elements of the cytoskeleton. Plakins, with their multi-domain structure and enormous size, not only play crucial roles in maintaining cell and tissue integrity and orchestrating dynamic changes in cytoarchitecture and cell shape, but also serve as scaffolding platforms for the assembly, positioning, and regulation of signaling complexes (reviewed in PMID: 9701547, 11854008, and 17499243). Plectin is expressed as several protein isoforms in a wide range of cell types and tissues from a single gene located on chromosome 8 in humans (PMID: 8633055, 8698233). Until 2010, this locus was named plectin 1 (symbol PLEC1 in human; Plec1 in mouse and rat) and the gene product had been referred to as "hemidesmosomal protein 1" or "plectin 1, intermediate filament binding 500kDa". These names were superseded by plectin. The plectin gene locus in mouse on chromosome 15 has been analyzed in detail (PMID: 10556294, 14559777), revealing a genomic exon-intron organization with well over 40 exons spanning over 62 kb and an unusual 5' transcript complexity of plectin isoforms. Eleven exons (1-1j) have been identified that alternatively splice directly into a common exon 2 which is the first exon to encode plectin's highly conserved actin binding domain (ABD). Three additional exons (-1, 0a, and 0) splice into an alternative first coding exon (1c), and two additional exons (2alpha and 3alpha) are optionally spliced within the exons encoding the acting binding domain (exons 2-8). Analysis of the human locus has identified eight of the eleven alternative 5' exons found in mouse and rat (PMID: 14672974); exons 1i, 1j and 1h have not been confirmed in human. Furthermore, isoforms lacking the central rod domain encoded by exon 31 have been detected in mouse (PMID:10556294), rat (PMID: 9177781), and human (PMID: 11441066, 10780662, 20052759). The short alternative amino-terminal sequences encoded by the different first exons direct the targeting of the various isoforms to distinct subcellular locations (PMID: 14559777). As the expression of specific plectin isoforms was found to be dependent on cell type (tissue) and stage of development (PMID: 10556294, 12542521, 17389230) it appears that each cell type (tissue) contains a unique set (proportion and composition) of plectin isoforms, as if custom-made for specific requirements of the particular cells. Concordantly, individual isoforms were found to carry out distinct and specific functions (PMID: 14559777, 12542521, 18541706). In 1996, a number of groups reported that patients suffering from epidermolysis bullosa simplex with muscular dystrophy (EBS-MD) lacked plectin expression in skin and muscle tissues due to defects in the plectin gene (PMID: 8698233, 8941634, 8636409, 8894687, 8696340). Two other subtypes of plectin-related EBS have been described: EBS-pyloric atresia (PA) and EBS-Ogna. For reviews of plectin-related diseases see PMID: 15810881, 19945614. Mutations in the plectin gene related to human diseases should be named based on the position in NM_000445 (variant 1, isoform 1c), unless the mutation is located within one of the other alternative first exons, in which case the position in the respective Reference Sequence should be used. [provided by RefSeq, Aug 2011]

Uniprot Description

Plectin-1: a major cytoskeleton crosslinking protein that binds to actin, intermediate filaments and microtubules. A scaffold for proteins involved in cellular signaling. Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the cross-linking and stabilization of cytoskeletal intermediate filaments network, but also in the regulation of their dynamics. Regulates the organization of keratin intermediate filaments (IF) and MAPK signaling during cell migration. Downregulates PKC signaling by sequestering RACK1. Redistributes to the uropod associated with vimentin and fodrin during polarization of peripheral blood T lymphocytes. This vimentin-plectin-fodrin complex provides a continuous linkage from the nucleus (lamin B) to the cortical cytoskeleton. Defects in PLEC1 are the cause of epidermolysis bullosa simplex 1 (EBS1). Three alternatively spliced isoforms have been described.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: brush border; costamere; cytoplasm; cytosol; focal adhesion; hemidesmosome; intermediate filament cytoskeleton; plasma membrane; sarcolemma; sarcoplasm

Molecular Function: actin binding; ankyrin binding; protein binding; structural constituent of muscle

Biological Process: hemidesmosome assembly