Rabbit anti-Human PLBD2 Polyclonal Antibody | anti-PLBD2 antibody

Anti-PLBD2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8107203

• Gene Names: PLBD2; P76
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: PLBD2; Polyclonal Antibody; Anti-PLBD2 Antibody; Rabbit Polyclonal; PLBD2 Antibody; Rabbit PAb; Antigen Affinity Purified; phospholipase B domain containing 2; Anti-P76 Antibody; anti-PLBD2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PLBD2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PLBD2 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:100-1:500; IP: 1-5uL/mg of lysate
• Immunogen: Recombinant Human PLBD2 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human PLBD2 (rh PLBD2). PLBD2 specific IgG was purified by Human PLBD2 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PLBD2 rabbit polyclonal antibody at 1:500 dilution Lane A: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:65 kDa Observed band size:70 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human PLBD2 in human brain with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(PLBD2 was immunoprecipitated using: Lane A:0.5 mg A549 Whole Cell Lysate 4 uL anti-PLBD2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PLBD2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 70 kDa Observed band size :70 kDa)

Related Product Information for anti-PLBD2 antibody

PLBD2 localizes to the lysosome, as its absence could plausibly lead to a serious yet unrecognized lysosomal storage disease. PLBD1 and PLBD2 are semi-orphans in the sense of being probable phospholipases of B class but with uncertain physiological substrates and thus functionalities. PLBD1 and PLBD2 constitute a small gene family (sequence homology class) within vertebrates though one that occurs expanded in some early diverging eukaryotes. PLBD2 presents a special difficulty in that a sequence of post-translational steps are apparently necessary for its activation. Without these, potential substrates can hardly be assayed. These steps include removal of the signal peptide, mannosylation appropriate to the lysosome targeting receptor, and self-catalytic proteolytic activation to expose the substrate binding site as this becomes appropriate.

References

Morgan CP. et al., 2004), Biochem J. 382 (2): 441-9. Kim W. et al., 2011, Mol Cell. 44 (2): 325-40. Havugimana PC. et al., 2012. Cell. 150 (5): 1068-81.

NCBI and Uniprot Product Information

• NCBI GI #: 229093316
• NCBI GeneID: 196463
• NCBI Accession #: NP_775813.2
• NCBI GenBank Nucleotide #: NM_173542.3
• UniProt Accession #: Q8NHP8; F5H5E2
• Molecular Weight: 61,885 Da
• NCBI Official Full Name: putative phospholipase B-like 2 isoform 1
• NCBI Official Synonym Full Names: phospholipase B domain containing 2
• NCBI Official Symbol: PLBD2
• NCBI Official Synonym Symbols: P76
• NCBI Protein Information: putative phospholipase B-like 2; PLB homolog 2; 76 kDa protein; LAMA-like protein 2; lamina ancestor homolog 2; phospholipase B-like 2 32 kDa form; phospholipase B-like 2 45 kDa form; phospholipase B domain-containing protein 2; mannose-6-phosphate protei
• UniProt Protein Name: Putative phospholipase B-like 2
• Protein Family: Putative phospholipase
• UniProt Gene Name: PLBD2
• UniProt Synonym Gene Names: p76
• UniProt Entry Name: PLBL2_HUMAN

Uniprot Description

PLBL2: Putative phospholipase. Belongs to the phospholipase B-like family.

Protein type: Phospholipase; EC 3.1.1.-

Chromosomal Location of Human Ortholog: 12q24.13

Cellular Component: lysosomal lumen

Molecular Function: hydrolase activity

Biological Process: lipid catabolic process