PLBL2/PLBD2 Recombinant Protein | PLBD2 recombinant protein

PLBL2/PLBD2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121594

• Gene Names: PLBD2; P76
• Purity: >95% as determined by SDS-PAGE
• Synonyms: PLBL2/PLBD2; PLBL2/PLBD2 Protein; Human; Recombinant (His Tag); Human PLBD2 Protein (His Tag); P76 Protein; phospholipase B domain containing 2; PLBD2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp589

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ile 42
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human PLBD2 (Q8NHP8)(Met1-Asp589) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PLBD2 recombinant protein

Background: PLBD2 localizes to the lysosome, as its absence could plausibly lead to a serious yet unrecognized lysosomal storage disease. PLBD1 and PLBD2 are semi-orphans in the sense of being probable phospholipases of B class but with uncertain physiological substrates and thus functionalities. PLBD1 and PLBD2 constitute a small gene family (sequence homology class) within vertebrates though one that occurs expanded in some early diverging eukaryotes. PLBD2 presents a special difficulty in that a sequence of post-translational steps are apparently necessary for its activation. Without these, potential substrates can hardly be assayed. These steps include removal of the signal peptide, mannosylation appropriate to the lysosome targeting receptor, and self-catalytic proteolytic activation to expose the substrate binding site as this becomes appropriate.

References

Morgan CP. et al., 2004), Biochem J. 382 (2): 441-9. Kim W. et al., 2011, Mol Cell. 44 (2): 325-40. Havugimana PC. et al., 2012. Cell. 150 (5): 1068-81.

NCBI and Uniprot Product Information

• NCBI GI #: 229093316
• NCBI GeneID: 196463
• NCBI Accession #: NP_775813.2
• NCBI GenBank Nucleotide #: NM_173542.3
• UniProt Accession #: Q8NHP8; F5H5E2
• Molecular Weight: 61,885 Da
• NCBI Official Full Name: putative phospholipase B-like 2 isoform 1
• NCBI Official Synonym Full Names: phospholipase B domain containing 2
• NCBI Official Symbol: PLBD2
• NCBI Official Synonym Symbols: P76
• NCBI Protein Information: putative phospholipase B-like 2; PLB homolog 2; 76 kDa protein; LAMA-like protein 2; lamina ancestor homolog 2; phospholipase B-like 2 32 kDa form; phospholipase B-like 2 45 kDa form; phospholipase B domain-containing protein 2; mannose-6-phosphate protei
• UniProt Protein Name: Putative phospholipase B-like 2
• Protein Family: Putative phospholipase
• UniProt Gene Name: PLBD2
• UniProt Synonym Gene Names: p76
• UniProt Entry Name: PLBL2_HUMAN

Uniprot Description

PLBL2: Putative phospholipase. Belongs to the phospholipase B-like family.

Protein type: Phospholipase; EC 3.1.1.-

Chromosomal Location of Human Ortholog: 12q24.13

Cellular Component: lysosomal lumen

Molecular Function: hydrolase activity

Biological Process: lipid catabolic process