Rabbit PLATELET FACTOR-4 Polyclonal Antibody | anti-PF4 antibody

RABBIT ANTI HUMAN PLATELET FACTOR-4

Cat. Num. AAA223824

• Gene Names: PF4; PF-4; CXCL4; SCYB4
• Applications: ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Synonyms: PLATELET FACTOR-4; Polyclonal Antibody; RABBIT ANTI HUMAN PLATELET FACTOR-4; anti-PF4 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0mg/ml after reconstitution (varies by lot)
• Sequence Length: 101

• Applicable Applications for anti-PF4 antibody: ELISA (EIA), Functional Assays (FN), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml;; Functional Assays: Minimum Dilution: 1.75; Maximum Dilution: 3.0ug/ml;; Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; ; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
• Immunogen: Recombinant human PF-4; Buffer Solution
• Antiserum Preparation: Antiserum to human PF-4 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Formalin fixed, paraffin embedded human breast malignant ductal adenocarcinoma stained with Rabbit anti Human platelet factor 4 (MBS223824) following heat mediated antigen retrieval with sodium citrate buffer (pH6.0) using an alkaline phosphatase conjugated polymer detection system and red chromogen)

Testing Data #2

(Formalin fixed, paraffin embedded human breast malignant ductal adenocarcinoma stained with Rabbit anti Human platelet factor 4 (MBS223824) following heat mediated antigen retrieval with sodium citrate buffer (pH6.0) using an alkaline phosphatase conjugated polymer detection system and red chromogen)

Testing Data #3

(Formalin fixed, paraffin embedded human breast malignant ductal adenocarcinoma stained with Rabbit anti Human platelet factor 4 (MBS223824) following heat mediated antigen retrieval with sodium citrate buffer (pH6.0) using an alkaline phosphatase conjugated polymer detection system and red chromogen)

Related Product Information for anti-PF4 antibody

Rabbit anti Human Platelet Factor-4 antibody specifically recognizes platelet factor-4 (PF-4), otherwise known as CXCL4, a member of the intercrine alpha (chemokine CXC) family, released from the alpha granules of activated platelets, during platelet aggregation. PF-4 binds with high affinity to heparin, counteracting its' anticoagulant effect, thereby promoting blood coagulation, and implicating PF-4 in wound repair. PF-4 acts as a chemotactic factor for neutrophils, monocytes and fibroblasts, and binds wih CXCR3B, a splice variant of the chemokine receptor CXCR3.

NCBI and Uniprot Product Information

• NCBI GI #: 4505733
• NCBI GeneID: 5196
• NCBI Accession #: NP_002610.1
• NCBI GenBank Nucleotide #: NM_002619.3
• UniProt Accession #: P02776; Q53X61; Q9UC64; Q9UC65
• Molecular Weight: 10,845 Da
• NCBI Official Full Name: platelet factor 4
• NCBI Official Synonym Full Names: platelet factor 4
• NCBI Official Symbol: PF4
• NCBI Official Synonym Symbols: PF-4; CXCL4; SCYB4
• NCBI Protein Information: platelet factor 4; C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4; iroplact; oncostatin-A
• UniProt Protein Name: Platelet factor 4
• Protein Family: Platelet factor
• UniProt Gene Name: PF4
• UniProt Synonym Gene Names: CXCL4; SCYB4; PF-4
• UniProt Entry Name: PLF4_HUMAN

NCBI Description

This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. The protein also exhibits antimicrobial activity against Plasmodium falciparum. [provided by RefSeq, Oct 2014]

Uniprot Description

PF4: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Chemokine; Apoptosis; Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q12-q21

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CXCR3 chemokine receptor binding; chemokine activity

Biological Process: negative regulation of cytolysis; platelet activation; cytokine and chemokine mediated signaling pathway; leukocyte chemotaxis; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of cAMP metabolic process; positive regulation of tumor necrosis factor production; negative regulation of megakaryocyte differentiation; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; platelet degranulation; negative regulation of MHC class II biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of macrophage differentiation; blood coagulation; inflammatory response