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Western Blot (WB) (Western Blot: Sample: Human MCF7 Cells.)

Rabbit anti-Rat Plasminogen Activator Inhibitor 2 (PAI2) Polyclonal Antibody | anti-PAI2 antibody

Polyclonal Antibody to Plasminogen Activator Inhibitor 2 (PAI2)

Gene Names
Serpinb2; Pai2a
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Plasminogen Activator Inhibitor 2 (PAI2); Polyclonal Antibody; Polyclonal Antibody to Plasminogen Activator Inhibitor 2 (PAI2); anti-PAI2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
The antibody is a rabbit polyclonal antibody raised against PAI2. It has been selected for its ability to recognize PAI2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- PEAVDFL ECANEARKKI NSWVKTQTKG EIPNLLPEGS VDEDTKMVLV NTIYFKGRWK TPFQKRLNGL YPFRVNLNES KPVQMMYLRE KLNIGYIKDL KTQILELPYI GNISMFLLLP DEIEDSSTGL EMLEREINFD NFNKWISKET LDEDDVLVYI PKFKLAQNYE LKPILQRMGM EDAFNKGKAD FSGMSESNDL FLSEVFHQAT VDVNEEGTVA AGGTGAVMTG RTGHGGPQFV ADHPFLFFIM NNITRTIL
Sequence Length
415
Applicable Applications for anti-PAI2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant PAI2 (Pro154~Leu408) expressed in E.coli.
Cross Reactivity
Human, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2043478
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Human MCF7 Cells.)

Western Blot (WB) (Western Blot: Sample: Human MCF7 Cells.)

Western Blot (WB)

(Western Blot: Sample: Recombinant PAI2, Rat.)

Western Blot (WB) (Western Blot: Sample: Recombinant PAI2, Rat.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,248 Da
NCBI Official Full Name
plasminogen activator inhibitor 2 type A
NCBI Official Synonym Full Names
serpin family B member 2
NCBI Official Symbol
Serpinb2
NCBI Official Synonym Symbols
Pai2a
NCBI Protein Information
plasminogen activator inhibitor 2 type A
UniProt Protein Name
Plasminogen activator inhibitor 2 type A
UniProt Gene Name
Serpinb2
UniProt Synonym Gene Names
Pai2; Planh2; PAI-2; PAI2A

NCBI Description

plays a role in proteolytic degradation of extracellular matrices in tissue remodeling; mediates the entire process of periodontal wound healing [RGD, Feb 2006]

Uniprot Description

Inhibits urokinase-type plasminogen activator.

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Product Notes

The PAI2 serpinb2 (Catalog #AAA2015539) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Plasminogen Activator Inhibitor 2 (PAI2) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Plasminogen Activator Inhibitor 2 (PAI2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PAI2 serpinb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- PEAVDFL ECANEARKKI NSWVKTQTKG EIPNLLPEGS VDEDTKMVLV NTIYFKGRWK TPFQKRLNGL YPFRVNLNES KPVQMMYLRE KLNIGYIKDL KTQILELPYI GNISMFLLLP DEIEDSSTGL EMLEREINFD NFNKWISKET LDEDDVLVYI PKFKLAQNYE LKPILQRMGM EDAFNKGKAD FSGMSESNDL FLSEVFHQAT VDVNEEGTVA AGGTGAVMTG RTGHGGPQFV ADHPFLFFIM NNITRTIL. It is sometimes possible for the material contained within the vial of "Plasminogen Activator Inhibitor 2 (PAI2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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