Typical Testing Data/Standard Curve (for reference only)
Rat HSP47 (Heat Shock Protein) CLIA Kit | HSP47 clia kit
Rat HSP47 (Heat Shock Protein) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to HSP47. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HSP47 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HSP47, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of HSP47. You can calculate the concentration of HSP47 in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Uniprot Description
HSP90A: a molecular chaperone of the heat shock protein 90 family. Has ATPase activity. Known to interact with a wide variety of proteins including steroid hormone receptors, neuropeptide Y, FKBP51/54, and FKBP52. G protein-coupled receptor kinases are stabilized by interacting with HSP 90. Hsp70 and Hsp90 promote tau solubility and tau binding to microtubules, reducing insoluble tau phosphorylation of tau.
Protein type: Heat shock protein; Chaperone
Chromosomal Location of Human Ortholog: 14q32.33
Cellular Component: cytoplasm; cytosol; extracellular region; lysosomal lumen; melanosome; membrane; myelin sheath; nucleoplasm; nucleus; plasma membrane
Molecular Function: ATP binding; ATPase activity; GTPase binding; histone deacetylase binding; identical protein binding; nitric-oxide synthase regulator activity; nucleotide binding; protein binding; protein homodimerization activity; TPR domain binding; unfolded protein binding
Biological Process: axon guidance; chaperone-mediated protein complex assembly; G2/M transition of mitotic cell cycle; innate immune response; mitochondrial transport; mitotic cell cycle; nitric oxide metabolic process; organelle organization and biogenesis; positive regulation of nitric oxide biosynthetic process; protein import into mitochondrial outer membrane; protein refolding; protein unfolding; receptor-mediated endocytosis; regulation of nitric-oxide synthase activity; regulation of protein complex assembly; regulation of protein ubiquitination; response to antibiotic; response to cold; response to heat; response to unfolded protein; signal transduction; vascular endothelial growth factor receptor signaling pathway
Research Articles on HSP47
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Product Notes
The Rat HSP47 hsp90aa1 (Catalog #AAA2531432) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2531432 CLIA Kit recognizes Rat HSP47. It is sometimes possible for the material contained within the vial of "HSP47 (Heat Shock Protein), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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