Rabbit anti-Human PLAGL1 / ZAC Polyclonal Antibody | anti-PLAGL1 / ZAC antibody

Anti-PLAGL1 / ZAC Antibody (aa311-360) IHC-plus

Cat. Num. AAA2400212

• Gene Names: PLAGL1; ZAC; LOT1; ZAC1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Immunoaffinity purified
• Synonyms: PLAGL1 / ZAC; Polyclonal Antibody; Anti-PLAGL1 / ZAC Antibody (aa311-360) IHC-plus; Rabbit Polyclonal (IgG) to Human PLAGL1 / ZAC; Human PLAGL1 / ZAC; Lost on transformation 1; LOT1; PLAG-like 1; LOT-1; ZAC tumor supressor; Zinc finger protein PLAGL1; Tumor supressor ZAC; ZAC; ZAC1; anti-PLAGL1 / ZAC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PLAGL1 Antibody detects endogenous levels of total PLAGL1 protein.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 463

• Applicable Applications for anti-PLAGL1 / ZAC antibody: Immunohistochemistry-Paraffin (IHC-P); Western Blot (WB); Peptide ELISA
• Application Notes: IHC-P (1:50); WB (1:500-1:1000); Peptide ELISA (1:10000); (Optimal dilution to be determined by the researcher.)
• Immunogen Species: PLAGL1 / ZAC antibody was raised against Human
• Immunogen: PLAGL1 / ZAC antibody was raised against synthetic peptide from human PLAGL1 (AA Range: 311-360).
• Epitope: aa311-360
• Target: PLAGL1 / ZAC
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-PLAGL1 / ZAC antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(Western blot of extracts from 293 cells, using PLAGL1 Antibody. The lane on the right is treated with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 27894293
• NCBI GeneID: 5325
• NCBI Accession #: NP_006709.2
• NCBI GenBank Nucleotide #: NM_006718.4
• UniProt Accession #: Q9UM63; O76019; Q7Z3V8; Q92981; Q96JR9; Q9UIZ0; B2RBA4; B2RCM8; E1P595; E1P597
• Molecular Weight: Western Blot: Predicted MW 50 kDa
• NCBI Official Full Name: zinc finger protein PLAGL1 isoform 2
• NCBI Official Synonym Full Names: PLAG1 like zinc finger 1
• NCBI Official Symbol: PLAGL1
• NCBI Official Synonym Symbols: ZAC; LOT1; ZAC1
• NCBI Protein Information: zinc finger protein PLAGL1
• UniProt Protein Name: Zinc finger protein PLAGL1
• UniProt Gene Name: PLAGL1
• UniProt Synonym Gene Names: LOT1; ZAC; LOT-1
• UniProt Entry Name: PLAL1_HUMAN

NCBI Description

This gene encodes a C2H2 zinc finger protein that functions as a suppressor of cell growth. This gene is often deleted or methylated and silenced in cancer cells. In addition, overexpression of this gene during fetal development is thought to be the causal factor for transient neonatal diabetes mellitus (TNDM). Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding two different protein isoforms. The P1 downstream promoter of this gene is imprinted, with preferential expression from the paternal allele in many tissues. [provided by RefSeq, Nov 2015]

Uniprot Description

PLAGL1: Shows weak transcriptional activatory activity. Transcriptional regulator of the type 1 receptor for pituitary adenylate cyclase-activating polypeptide. Belongs to the krueppel C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 6q24-q25

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; nucleoplasm; nucleus

Molecular Function: DNA binding; metal ion binding

Biological Process: apoptosis; cell cycle arrest; cell differentiation; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter

Disease: Diabetes Mellitus, Transient Neonatal, 1