Rabbit anti-Human EIF2AK2 / PKR Polyclonal Antibody | anti-EIF2AK2 antibody

Rabbit Polyclonal (IgG) to Human EIF2AK2 / PKR

Cat. Num. AAA244663

• Gene Names: EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: EIF2AK2 / PKR; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human EIF2AK2 / PKR; Anti-EIF2AK2 / PKR Antibody (aa418-467) IHC-plus; EIF2AK2; p1/eIF-2A protein kinase; p68 kinase; PRKR; EIF-2A protein kinase 2; p68; Protein kinase RNA-activated; Tik; Human EIF2AK2; PKR; anti-EIF2AK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PKR (Ab-451) Antibody detects endogenous levels of total PKR protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 551

• Applicable Applications for anti-EIF2AK2 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:10000), IHC-P (10 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human EIF2AK2 (aa418-467).
• Immunogen: EIF2AK2 / PKR antibody was raised against synthetic peptide from human EIF2AK2 (aa418-467).
• Antigen Modification: aa418-467
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-EIF2AK2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Related Product Information for anti-EIF2AK2 antibody

PRKR is a Translational Control type protein kinase. PRKR, induced by interferon (IFN) and activated by double-stranded RNA in the presence of ATP, inhibits translation by phosphorylating the initiation factor eIF2alpha chain. PRKR activation has been linked to the Huntington disease process.

Product Categories/Family for anti-EIF2AK2 antibody

Family: Protein Kinase; Subfamily: Translational Control Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 4506103
• NCBI GeneID: 5610
• NCBI Accession #: NP_002750.1
• NCBI GenBank Nucleotide #: NM_002759.3
• UniProt Accession #: P19525; Q52M43; Q7Z6F6; Q9UIR4; A8K3P0; D6W584; E9PC80
• Molecular Weight: 57,391 Da
• NCBI Official Full Name: interferon-induced, double-stranded RNA-activated protein kinase isoform a
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2-alpha kinase 2
• NCBI Official Symbol: EIF2AK2
• NCBI Official Synonym Symbols: PKR; PRKR; EIF2AK1; PPP1R83
• NCBI Protein Information: interferon-induced, double-stranded RNA-activated protein kinase; p68 kinase; eIF-2A protein kinase 2; P1/eIF-2A protein kinase; tyrosine-protein kinase EIF2AK2; interferon-inducible elF2alpha kinase; double stranded RNA activated protein kinase; protein phosphatase 1, regulatory subunit 83; protein kinase, interferon-inducible double stranded RNA dependent
• UniProt Protein Name: Interferon-induced, double-stranded RNA-activated protein kinase
• Protein Family: Interferon-induced, double-stranded RNA-activated protein kinase
• UniProt Gene Name: EIF2AK2
• UniProt Synonym Gene Names: eIF-2A protein kinase 2; PKR; Protein kinase R
• UniProt Entry Name: E2AK2_HUMAN

NCBI Description

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

PKR: a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis. Controls the activation of several transcription factors such as NF-kappaB, p53 and Stats. Mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation.

Protein type: Protein kinase, Other; EC 2.7.10.2; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Translation; Other group; PEK family

Chromosomal Location of Human Ortholog: 2p22-p21

Cellular Component: membrane; perinuclear region of cytoplasm; cytoplasm; ribosome; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; double-stranded RNA binding; non-membrane spanning protein tyrosine kinase activity; protein phosphatase type 2A regulator activity; eukaryotic translation initiation factor 2alpha kinase activity; ATP binding; protein kinase activity

Biological Process: positive regulation of cytokine production; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; translation; transcription, DNA-dependent; unfolded protein response; response to virus; protein amino acid autophosphorylation; viral infectious cycle; protein amino acid phosphorylation; positive regulation of chemokine production; positive regulation of stress-activated MAPK cascade; evasion by virus of host immune response; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; negative regulation of viral genome replication; modification by virus of host cellular process; negative regulation of translation; virus-host interaction; innate immune response; negative regulation of osteoblast proliferation; defense response to virus; negative regulation of apoptosis