Rabbit anti-Human PKD/PKCu Polyclonal Antibody | anti-PKD/PKCu antibody

PKD/PKCu (Phospho-Ser910) Antibody

Cat. Num. AAA9401452

• Gene Names: PRKD1; PKD; PKCM; PRKCM; PKC-MU
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: PKD/PKCu; Polyclonal Antibody; PKD/PKCu (Phospho-Ser910) Antibody; KPCD1; PKC-mu; PKCM; PKD; PRKCM; anti-PKD/PKCu antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of PKD/PKCm only when phosphorylated at serine 910.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 912

• Applicable Applications for anti-PKD/PKCu antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:1000; Immunofluorescence: 1:100~1:200
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine 910 (R-V-S(p)-I-L) derived from Human PKD/PKCm.
• Modification: Phospho-Ser910
• Target Name: PKD/PKCm
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from EGF-treated 293 and PMA-treated HUVEC cells using PKD/PKCm(Phospho-Ser910) Antibody.)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using PKD/PKCm(Phospho-Ser910) Antibody.)

Related Product Information for anti-PKD/PKCu antibody

Converts transient diacylglycerol. (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.

Product Categories/Family for anti-PKD/PKCu antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 115529463
• NCBI GeneID: 5587
• NCBI Accession #: NP_002733.2
• NCBI GenBank Nucleotide #: NM_002742.2
• UniProt Accession #: Q15139; A6NL64; B2RAF6
• Molecular Weight: 101,704 Da
• NCBI Official Full Name: serine/threonine-protein kinase D1
• NCBI Official Synonym Full Names: protein kinase D1
• NCBI Official Symbol: PRKD1
• NCBI Official Synonym Symbols: PKD; PKCM; PRKCM; PKC-MU
• NCBI Protein Information: serine/threonine-protein kinase D1
• UniProt Protein Name: Serine/threonine-protein kinase D1
• UniProt Gene Name: PRKD1
• UniProt Synonym Gene Names: PKD; PKD1; PRKCM
• UniProt Entry Name: KPCD1_HUMAN

NCBI Description

PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion (summary by Eiseler et al., 2009 [PubMed 19329994]).[supplied by OMIM, Nov 2010]

Uniprot Description

PRKD1: a CAMK kinase of the PKD family. Cleavage by caspase-3 following DNA damage activates it and alters its subcellular localization. Sensitizes cells to apoptosis induced by genotoxic stress. Its cleavage is blocked in cells that over-express the anti-apoptotic Bcl-x(L) protein. Expression of a caspase-resistant mutant partially inhibits DNA damage-induced apoptosis. Its activation by TLR ligands is dependent on MyD88, IRAK4 and -1, but not TRAF6. Essential for MyD88-dependent proinflammatory immune responses. Activated by diacylglycerol and phorbol esters. Binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface. Colocalizes with F-actin at peripheral F-actin-rich structures in membrane ruffles at the edge of lamellipodia in cervical carcinoma cells. Substrates reportedly include critical regulatory proteins including CREB, SSH1L, CTNNB1, HDACs 5 and 7, PKD1, HPK1, MARK2, PIP5K2A, PPP1R14A

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; EC 2.7.11.13; Autophagy; Protein kinase, CAMK; CAMK group; PKD family

Chromosomal Location of Human Ortholog: 14q11

Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; intercellular junction; trans-Golgi network; cell cortex; cytosol; nucleus

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; protein kinase C activity; metal ion binding; ATP binding

Biological Process: integrin-mediated signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; sphingolipid metabolic process; sphingolipid biosynthetic process; apoptosis; protein amino acid autophosphorylation; negative regulation of endocytosis; positive regulation of blood vessel endothelial cell migration; activation of CREB transcription factor; signal transduction; positive regulation of peptidyl-serine phosphorylation; Golgi vesicle transport; activation of NF-kappaB transcription factor; positive regulation of osteoblast differentiation; cell proliferation; positive regulation of angiogenesis; peptidyl-serine phosphorylation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; angiogenesis; inflammatory response; vascular endothelial growth factor receptor signaling pathway; Golgi organization and biogenesis