Western Blot (WB)
(Staining (1ug/ml) of human muscle lysate (RIPA buffer, 35ug total)
Goat MURF3/TRIM54 Polyclonal Antibody | anti-TRIM54 antibody
Goat anti-MURF3/TRIM54 Antibody
Gene Names
TRIM54; MURF; RNF30; muRF3; MURF-3
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
MURF3/TRIM54; Polyclonal Antibody; Goat anti-MURF3/TRIM54 Antibody; TRIM54; MURF-3; tripartite motif-containing 54; RNF30; MURF; ring finger protein 30; muscle-specific RING-finger protein 3; muscle-specific RING-finger protein homolog; OTTHUMP00000122598; TRIM54 antibody; MURF-3 antibody; tripartite motif-containing 54 antibody; RNF30 antibody; MURF antibody; ring finger protein 30 antibody; muscle-specific RING-finger protein 3 antibody; muscle-specific RING-finger protein homolog antibody; OTTHUMP00000122598 antibody; MURF3 / TRIM54; anti-TRIM54 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
GSAGPEEERPDGP
Sequence Length
400
Applicable Applications for anti-TRIM54 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Experiments gave bands at approx 45kDa and 38kDa in human skeletal muscle lysate after 1ug/ml antibody staining. These bands correspond to the reported isoforms with calculated MWs of 43.1kDa according to NP_115935, and 38.6kDa according to NP_912730. Recommended concentration: 1-3ug/ml.
Western Blot: Experiments gave bands at approx 45kDa and 38kDa in human skeletal muscle lysate after 1ug/ml antibody staining. These bands correspond to the reported isoforms with calculated MWs of 43.1kDa according to NP_115935, and 38.6kDa according to NP_912730. Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-GSAGPEEERPDGP, from the C Terminus of the protein sequence according to NP_115935.3; NP_912730.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
(Staining (1ug/ml) of human muscle lysate (RIPA buffer, 35ug total)
Western Blot (WB)
(Staining (1ug/ml) of human muscle lysate (RIPA buffer, 35ug total)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,833 Da
NCBI Official Full Name
tripartite motif-containing protein 54 isoform 1
NCBI Official Synonym Full Names
tripartite motif containing 54
NCBI Official Symbol
TRIM54
NCBI Official Synonym Symbols
MURF; RNF30; muRF3; MURF-3
NCBI Protein Information
tripartite motif-containing protein 54
UniProt Protein Name
Tripartite motif-containing protein 54
Protein Family
UniProt Gene Name
TRIM54
UniProt Synonym Gene Names
MURF; MURF3; RNF30; MuRF; MuRF-3; MuRF3
UniProt Entry Name
TRI54_HUMAN
NCBI Description
The protein encoded by this gene contains a RING finger motif and is highly similar to the ring finger proteins RNF28/MURF1 and RNF29/MURF2. In vitro studies demonstrated that this protein, RNF28, and RNF29 form heterodimers, which may be important for the regulation of titin kinase and microtubule-dependent signal pathways in striated muscles. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
TRIM54: May bind and stabilize microtubules during myotubes formation. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.3.2.-; Ligase; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 2p23.3
Cellular Component: microtubule; microtubule associated complex; Z disc
Molecular Function: microtubule binding; protein binding; signal transducer activity; zinc ion binding
Biological Process: cell differentiation; microtubule-based process; multicellular organismal development; negative regulation of microtubule depolymerization; signal transduction
Research Articles on TRIM54
Similar Products
Product Notes
The TRIM54 trim54 (Catalog #AAA421339) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MURF3/TRIM54 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's MURF3/TRIM54 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Experiments gave bands at approx 45kDa and 38kDa in human skeletal muscle lysate after 1ug/ml antibody staining. These bands correspond to the reported isoforms with calculated MWs of 43.1kDa according to NP_115935, and 38.6kDa according to NP_912730. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the TRIM54 trim54 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GSAGPEEERP DGP. It is sometimes possible for the material contained within the vial of "MURF3/TRIM54, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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