Western Blot (WB)
(Western Blot analysis in NCI-H460 cell lysate usingMBS644425. PKCnu (arrow) was detected usingMBS644425. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Rabbit anti-Human PKC nu Polyclonal Antibody | anti-PRKCN antibody
PKC nu, CT (Serine/Threonine-protein Kinase D3, Protein Kinase C, nu Type, nPKC-nu, Protein Kinase EPK2, PRKD3, PRKCN, EPK2) (PE)
Gene Names
PRKD3; EPK2; PKD3; PRKCN; PKC-NU; nPKC-NU
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Immunofluorescence
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
PKC nu; Polyclonal Antibody; CT (Serine/Threonine-protein Kinase D3; Protein Kinase C; nu Type; nPKC-nu; Protein Kinase EPK2; PRKD3; PRKCN; EPK2) (PE); anti-PRKCN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PKC nu.
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-PRKCN antibody
Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB), Immunofluorescence (IF)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 860-890 in the C-terminal region of human PKC nu. Species sequence homology: mouse
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in NCI-H460 cell lysate usingMBS644425. PKCnu (arrow) was detected usingMBS644425. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(Western Blot analysis in NCI-H460 cell lysate usingMBS644425. PKCnu (arrow) was detected usingMBS644425. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS644425, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of MBS644425 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS644425, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of MBS644425 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB)
(Upper panel, Western Blot analysis of GFP fusion protein expression in Panc-1 cells by using an anti-GFP antibody. Lanes 1 and 5: non-transfected cells; lanes 2 and 6: GFP-PKD-transfected cells; lanes 3 and 7: GFP-PKD2-transfected cells; lanes 4 and 8: GFP-PKD3 transfected cells. Center panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using 040093 andMBS644425. Lower panel, indirect immunofluorescence analysis of GFP-PKD3 fusion protein expression in Panc-1 cells by using 040093 andMBS644425. Data courtesy of Dr. Osvaldo Rey, University of California Los Angeles.)
Western Blot (WB)
(Upper panel, Western Blot analysis of GFP fusion protein expression in Panc-1 cells by using an anti-GFP antibody. Lanes 1 and 5: non-transfected cells; lanes 2 and 6: GFP-PKD-transfected cells; lanes 3 and 7: GFP-PKD2-transfected cells; lanes 4 and 8: GFP-PKD3 transfected cells. Center panel, western blot analysis of GFP fusion protein expression in Panc-1 cells by using 040093 andMBS644425. Lower panel, indirect immunofluorescence analysis of GFP-PKD3 fusion protein expression in Panc-1 cells by using 040093 andMBS644425. Data courtesy of Dr. Osvaldo Rey, University of California Los Angeles.)
Product Categories/Family for anti-PRKCN antibody
References
1. Yeaman, C., et al., Nat. Cell Biol. 6(2):106-112 (2004). 2. Rey, O., et al., J. Biol. Chem. 278(26):23773-23785 (2003). 3. Matthews, S.A., et al., J. Biol. Chem. 278(11):9086-9091 (2003). 4. Bennasser, Y., et al., Virology 303(1):174-180 (2002). 5. Bennasser, Y., et al., FASEB J. 16(6):546-554 (2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,123 Da
NCBI Official Full Name
serine/threonine-protein kinase D3
NCBI Official Synonym Full Names
protein kinase D3
NCBI Official Symbol
PRKD3
NCBI Official Synonym Symbols
EPK2; PKD3; PRKCN; PKC-NU; nPKC-NU
NCBI Protein Information
serine/threonine-protein kinase D3; protein kinase EPK2; protein kinase C, nu; protein kinase C nu type; protein-serine/threonine kinase
UniProt Protein Name
Serine/threonine-protein kinase D3
UniProt Gene Name
PRKD3
UniProt Synonym Gene Names
EPK2; PRKCN
UniProt Entry Name
KPCD3_HUMAN
Similar Products
Product Notes
The PRKCN prkd3 (Catalog #AAA6495201) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PKC nu, CT (Serine/Threonine-protein Kinase D3, Protein Kinase C, nu Type, nPKC-nu, Protein Kinase EPK2, PRKD3, PRKCN, EPK2) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PKC nu can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB), Immunofluorescence (IF). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PRKCN prkd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKC nu, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
