Rabbit PKC epsilon Polyclonal Antibody | anti-PKC epsilon antibody

Rabbit anti PKC epsilon Polyclonal Antibody

Cat. Num. AAA460181

• Gene Names: PRKCE; PKCE; nPKC-epsilon
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Epitope Affinity Purification
• Synonyms: PKC epsilon; Polyclonal Antibody; Rabbit anti PKC epsilon Polyclonal Antibody; protein kinase C; epsilon; PKCE; anti-PKC epsilon antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. Protein kinase C epsilon is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, insulin exocytosis, It is physically associated with the phosphorylation of cytokeratins 8/18.
• Purity/Purification: Purified by Epitope Affinity Purification
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 737

• Applicable Applications for anti-PKC epsilon antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Flow Cytometry: 5-10 ug/ml; Immunohistochemistry: 2-10 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation: A synthetic peptide corresponding to C-term of human PKC epsilon. This sequence is identical to mouse and rat.
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Related Product Information for anti-PKC epsilon antibody

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. Protein kinase C epsilon is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, insulin exocytosis, It is physically associated with the phosphorylation of cytokeratins 8/18.

References

Omary, M.B., Baxter, G.T., Chou, C.F., Riopel, C.L., Lin, W.Y. and Strulovici, B. PKC epsilon-related kinase associates with and phosphorylates cytokeratin 8 and 18. J. Cell Biol. 117 (3), 583-593 (1992)

NCBI and Uniprot Product Information

• NCBI GI #: 4885563
• NCBI GeneID: 5581
• NCBI Accession #: NP_005391.1
• NCBI GenBank Nucleotide #: NM_005400.2
• UniProt Accession #: Q02156; Q32MQ3; Q53SL4; Q53SM5; Q9UE81; B0LPH7
• Molecular Weight: 83,674 Da
• NCBI Official Full Name: protein kinase C epsilon type
• NCBI Official Synonym Full Names: protein kinase C epsilon
• NCBI Official Symbol: PRKCE
• NCBI Official Synonym Symbols: PKCE; nPKC-epsilon
• NCBI Protein Information: protein kinase C epsilon type
• UniProt Protein Name: Protein kinase C epsilon type
• UniProt Gene Name: PRKCE
• UniProt Synonym Gene Names: PKCE

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCE: an AGC kinase of the PKC family. Calcium-independent, phospholipid-dependent. Activated by inflammatory mediators and involved in nociceptive functions. PKC-epsilon null mice display decreased hypoxic pulmonary vasoconstriction.

Protein type: AGC group; EC 2.7.11.13; Eta subfamily; Kinase, protein; PKC family; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; Golgi apparatus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: actin monomer binding; enzyme activator activity; enzyme binding; protein binding; protein kinase C activity; protein serine/threonine kinase activity; signal transducer activity

Biological Process: apoptosis; lipopolysaccharide-mediated signaling pathway; peptidyl-serine phosphorylation; phospholipase C activation; platelet activation; positive regulation of actin filament polymerization; positive regulation of cytokinesis; protein amino acid phosphorylation; signal transduction