Rabbit anti-Human, Mouse PKC alpha Polyclonal Antibody | anti-PKC alpha antibody

PKC alpha Rabbit Polyclonal

Cat. Num. AAA7602713

• Gene Names: PRKCA; PKRCA
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: PKC alpha; Polyclonal Antibody; PKC alpha Rabbit Polyclonal; anti-PKC alpha antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 672

• Applicable Applications for anti-PKC alpha antibody: ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
• Immunogen: Protein kinase C, alpha
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human thyroid cancer tissue slide using MBS7602713 (PRKCA Antibody) at dilution of 1:200)

Immunofluorescence (IF)

(Immunofluorescent analysis of (-20 degree C Ethanol) fixed HeLa cells using MBS7602713 (PRKCA Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L))

Western Blot (WB)

(Jurkat cells were subjected to SDS PAGE followed by western blot with MBS7602713 (PRKCA antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 27806089
• NCBI GeneID: 282001
• NCBI Accession #: NP_776860.1
• NCBI GenBank Nucleotide #: NM_174435.1
• Molecular Weight: 76,837 Da
• NCBI Official Full Name: protein kinase C alpha type
• NCBI Official Symbol: PRKCA
• NCBI Official Synonym Symbols: PKRCA
• NCBI Protein Information: protein kinase C alpha type
• UniProt Protein Name: Protein kinase C alpha type
• UniProt Gene Name: PRKCA
• UniProt Synonym Gene Names: PKC-A; PKC-alpha
• UniProt Entry Name: KPCA_BOVIN

Uniprot Description

PKCA: an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters. Contains a pseudo-substrate autoinhibitory domain that binds to the catalytic domain preventing its activation in the absence of cofactors or activators.

Protein type: EC 2.7.11.13; Protein kinase, AGC; Oncoprotein; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; PKC family; Alpha subfamily

Cellular Component: cytoplasm; cytosol; intracellular; mitochondrial membrane; nucleus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: ATP binding; protein binding; protein kinase activity; protein kinase C activity; zinc ion binding

Biological Process: adenylate cyclase activation; angiogenesis; apoptosis; cell adhesion; negative regulation of adenylate cyclase activity; peptidyl-serine phosphorylation; positive regulation of angiogenesis; positive regulation of cell adhesion; positive regulation of cell migration; positive regulation of endothelial cell proliferation; positive regulation of lipopolysaccharide-mediated signaling pathway; positive regulation of macrophage differentiation; positive regulation of mitotic cell cycle; protein amino acid phosphorylation; regulation of rhodopsin mediated signaling