Western Blot (WB)
(Western blot analysis of PKA C beta expression in HEK293T (A), NIH3T3 (B), mouse lung (C), rat lung (D) whole cell lysates.)
Rabbit PKA C beta Polyclonal Antibody | anti-PRKACB antibody
Anti-PKA C beta Antibody
Western Blot (WB)
(Western blot analysis of PKA C beta expression in HEK293T (A), NIH3T3 (B), mouse lung (C), rat lung (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of PKA C beta expression in HEK293T (A), NIH3T3 (B), mouse lung (C), rat lung (D) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PKA C beta staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PKA C beta staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the serine/threonine protein kinase family. The encoded protein is a catalytic subunit of cAMP (cyclic AMP)-dependent protein kinase, which mediates signalling though cAMP. cAMP signaling is important to a number of processes, including cell proliferaton and differentiation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2014]
Uniprot Description
PKACB: Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. The cAMP-dependent protein kinase catalytic subunit binds PJA2. Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain. Activated by cAMP. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. 9 isoforms of the human protein are produced by alternative splicing.
Protein type: Protein kinase, AGC; EC 2.7.11.11; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; PKA family
Chromosomal Location of Human Ortholog: 1p31.1
Cellular Component: nucleoplasm; centrosome; perinuclear region of cytoplasm; plasma membrane; cytosol; cAMP-dependent protein kinase complex
Molecular Function: protein binding; cAMP-dependent protein kinase activity; ubiquitin protein ligase binding; magnesium ion binding; ATP binding
Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; activation of protein kinase A; glucose metabolic process; pathogenesis; negative regulation of meiotic cell cycle; signal transduction; protein amino acid phosphorylation; gluconeogenesis; G-protein signaling, coupled to cAMP nucleotide second messenger; synaptic transmission; phospholipase C activation; neural tube closure; triacylglycerol catabolic process; carbohydrate metabolic process; energy reserve metabolic process; renal water homeostasis; innate immune response; blood coagulation; transmembrane transport; regulation of insulin secretion
Research Articles on PRKACB
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Product Notes
The PRKACB prkacb (Catalog #AAA820292) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PKA C beta Antibody reacts with Human, Mouse, Rat, Bovine, Chicken, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's PKA C beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000), IH (1/100 - 1/200). Researchers should empirically determine the suitability of the PRKACB prkacb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKA C beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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