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(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKA CAT (Phospho-Thr197) Antibody)
Rabbit PKA alpha/beta/gamma Polyclonal Antibody | anti-PRKACA/PRKACB antibody
phospho-PKA alpha/beta/gamma (catalytic subunit) (Thr197) Rabbit pAb
Gene Names
PRKACA; PKACA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, ELISA
Purity
Affinity Chromatography
Synonyms
PKA alpha/beta/gamma; Polyclonal Antibody; phospho-PKA alpha/beta/gamma (catalytic subunit) (Thr197) Rabbit pAb; PRKACA; PKACA; cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PRKACB; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta; PRKACG; cAMP-dependent protein kinase catalytic subunit gamma; PKA C-gamma; anti-PRKACA/PRKACB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-PKAalpha/beta/gamma cat (T198) Polyclonal Antibody detects endogenous levels of PKAalpha/beta/gamma cat protein only when phosphorylated at T198.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applicable Applications for anti-PRKACA/PRKACB antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:100-1:300
IF: 1:200-1:1000
ELISA: 1:10,000
IHC: 1:100-1:300
IF: 1:200-1:1000
ELISA: 1:10,000
Immunogen
The antiserum was produced against synthesized peptide derived from human PKA CAT around the phosphorylation site of Thr197. AA range:166-215
Conjugation
Unconjugated
Modification
Phosphoralated
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKA CAT (Phospho-Thr197) Antibody)
(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKA CAT (Phospho-Thr197) Antibody)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells, using PKA CAT (Phospho-Thr197) Antibody. The picture on the right is blocked with the phospho peptide.)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells, using PKA CAT (Phospho-Thr197) Antibody. The picture on the right is blocked with the phospho peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PKA CAT (Phospho-Thr197) Antibody. The picture on the right is blocked with the phospho peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PKA CAT (Phospho-Thr197) Antibody. The picture on the right is blocked with the phospho peptide.)
Western Blot (WB)
(Western blot analysis of lysates from mouse brain, using PKA CAT (Phospho-Thr197) Antibody. The lane on the right is blocked with the phospho peptide.)
Western Blot (WB)
(Western blot analysis of lysates from mouse brain, using PKA CAT (Phospho-Thr197) Antibody. The lane on the right is blocked with the phospho peptide.)
Related Product Information for anti-PRKACA/PRKACB antibody
PRKACA (protein kinase cAMP-activated catalytic subunit alpha) encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.
Product Categories/Family for anti-PRKACA/PRKACB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
351
NCBI Official Full Name
cAMP-dependent protein kinase catalytic subunit alpha isoform 1
NCBI Official Synonym Full Names
protein kinase, cAMP-dependent, catalytic, alpha
NCBI Official Symbol
PRKACA
NCBI Official Synonym Symbols
PKACA
NCBI Protein Information
cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; protein kinase A catalytic subunit; cAMP-dependent protein kinase catalytic subunit alpha, isoform 1
UniProt Protein Name
cAMP-dependent protein kinase catalytic subunit alpha
UniProt Gene Name
PRKACA
UniProt Synonym Gene Names
PKACA; PKA C-alpha
UniProt Entry Name
KAPCA_HUMAN
Similar Products
Product Notes
The PRKACA/PRKACB prkaca (Catalog #AAA4755399) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The phospho-PKA alpha/beta/gamma (catalytic subunit) (Thr197) Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PKA alpha/beta/gamma can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:100-1:300 IF: 1:200-1:1000 ELISA: 1:10,000. Researchers should empirically determine the suitability of the PRKACA/PRKACB prkaca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKA alpha/beta/gamma, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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