Rabbit PIWIL4 Polyclonal Antibody | anti-PIWIL4 antibody

PIWIL4 (Piwi-like Protein 4, HIWI2, MIWI2) (HRP)

Cat. Num. AAA6431747

• Gene Names: PIWIL4; HIWI2; MIWI2
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: PIWIL4; Polyclonal Antibody; PIWIL4 (Piwi-like Protein 4; HIWI2; MIWI2) (HRP); anti-PIWIL4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes PIWIL4. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-PIWIL4 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human PIWIL4.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PIWIL4 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cell using PIWIL4 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-PIWIL4 antibody

Antibodies; RNA Processing

NCBI and Uniprot Product Information

• NCBI GI #: 116242716
• NCBI GeneID: 143689
• UniProt Accession #: Q7Z3Z4; Q68CZ4; Q8N8G9; Q8N9V8; Q8NEH2; B4DEG5
• Molecular Weight: 852
• NCBI Official Full Name: Piwi-like protein 4
• NCBI Official Synonym Full Names: piwi-like RNA-mediated gene silencing 4
• NCBI Official Symbol: PIWIL4
• NCBI Official Synonym Symbols: HIWI2; MIWI2
• NCBI Protein Information: piwi-like protein 4; piwi-like 4
• UniProt Protein Name: Piwi-like protein 4
• Protein Family: Piwi-like protein
• UniProt Gene Name: PIWIL4
• UniProt Synonym Gene Names: HIWI2; PIWI
• UniProt Entry Name: PIWL4_HUMAN

NCBI Description

PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM, Mar 2008]

Uniprot Description

PIWIL4: Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci. Belongs to the argonaute family. Piwi subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: cytoplasm; nucleus

Biological Process: regulation of translation; meiotic cell cycle; DNA methylation during gametogenesis; multicellular organismal development; spermatogenesis; RNA-mediated gene silencing; gene expression; cell differentiation