Rabbit anti-Human PIWIL2 Polyclonal Antibody | anti-PIWIL2 antibody

PIWIL2, NT (PIWIL2, HILI, Piwi-like protein 2, Cancer/testis antigen 80) (Biotin)

Cat. Num. AAA6334280

• Gene Names: PIWIL2; CT80; HILI; mili; PIWIL1L
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PIWIL2; Polyclonal Antibody; NT (PIWIL2; HILI; Piwi-like protein 2; Cancer/testis antigen 80) (Biotin); Cancer/testis antigen 80; anti-PIWIL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PIWIL2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PIWIL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-119 amino acids from the N-terminal region of human PIWIL2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PIWIL2 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PIWIL2 antibody detected the PIWIL2 protein (arrow).)

Related Product Information for anti-PIWIL2 antibody

PIWIL2 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).

Product Categories/Family for anti-PIWIL2 antibody

Antibodies; Cancer Markers

References

Lee, J.H., et al. Cancer Res. 70(11):4569-4579(2010). Liu, J.J., et al. Int J Clin Exp Pathol 3(4):328-337(2010). Nikpour, P., et al. Cancer Epidemiol 33 (3-4), 271-275 (2009): Lee, J.H., et al. Hum. Mol. Genet. 15(2):201-211(2006). Kuramochi-Miyagawa, S., et al. Development 131(4):839-849(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 24431985
• NCBI GeneID: 55124
• NCBI Accession #: NP_060538.2
• NCBI GenBank Nucleotide #: NM_018068.3
• UniProt Accession #: Q8TC59; Q96SW6; Q9NW28; A8K4S3; A8K8S5; B0AZN9; B0AZP2; B4DR22; E7ECA4
• Molecular Weight: 105,760 Da
• NCBI Official Full Name: piwi-like protein 2
• NCBI Official Synonym Full Names: piwi-like RNA-mediated gene silencing 2
• NCBI Official Symbol: PIWIL2
• NCBI Official Synonym Symbols: CT80; HILI; mili; PIWIL1L
• NCBI Protein Information: piwi-like protein 2; Miwi like; piwi-like 2; piwil2-like protein; cancer/testis antigen 80; 80kDa PIWIL2 short isoform
• UniProt Protein Name: Piwi-like protein 2
• Protein Family: Piwi-like protein
• UniProt Gene Name: PIWIL2
• UniProt Synonym Gene Names: HILI; CT80
• UniProt Entry Name: PIWL2_HUMAN

NCBI Description

PIWIL2 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM, Mar 2008]

Uniprot Description

PIWIL2: Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors. Interacts with DDX4, MAEL, EIF3A, EIF4E, EIF4G, PRMT5 and WDR77. Associates with EIF4E- and EIF4G-containing m7G cap-binding complexes. Interacts (when methylated on arginine residues) with TDRD1. Expressed in adult testis and in most tumors. Belongs to the argonaute family. Piwi subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cancer Testis Antigen (CTA); Apoptosis

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: polysome; cytoplasm

Molecular Function: mRNA binding

Biological Process: oogenesis; positive regulation of translation; meiotic cell cycle; DNA methylation during gametogenesis; multicellular organismal development; RNA-mediated gene silencing; germ-line stem cell maintenance; gene expression; spermatogenesis