Western Blot (WB)
(Staining (1ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat PITPN/PITP alpha Polyclonal Antibody | anti-PITPN antibody
Goat anti-PITPN/PITP alpha Antibody
Gene Names
PITPNA; PITPN; VIB1A; HEL-S-36; PI-TPalpha
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
PITPN/PITP alpha; Polyclonal Antibody; Goat anti-PITPN/PITP alpha Antibody; PITPN; PITP alpha; PITPNB; phosphotidylinositol transfer protein; phosphatidylinositol transfer protein; PITPN antibody; PITP alpha antibody; PITPNB antibody; phosphotidylinositol transfer protein antibody; phosphatidylinositol transfer protein antibody; PITPN / PITP alpha; anti-PITPN antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RQKDPVKGMTADD
Sequence Length
270
Applicable Applications for anti-PITPN antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Approx 32kDa band observed in 293 and Human Liver lysates (predicted MW of 31kDa according to NP_006215). Recommended for use at 1-3ug/ml.
Western Blot: Approx 32kDa band observed in 293 and Human Liver lysates (predicted MW of 31kDa according to NP_006215). Recommended for use at 1-3ug/ml.
Immunogen
Peptide with sequence C-RQKDPVKGMTADD, from the C Terminus of the protein sequence according to NP_006215.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
(Staining (1ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (1ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,806 Da
NCBI Official Full Name
phosphatidylinositol transfer protein alpha isoform
NCBI Official Synonym Full Names
phosphatidylinositol transfer protein alpha
NCBI Official Symbol
PITPNA
NCBI Official Synonym Symbols
PITPN; VIB1A; HEL-S-36; PI-TPalpha
NCBI Protein Information
phosphatidylinositol transfer protein alpha isoform
UniProt Protein Name
Phosphatidylinositol transfer protein alpha isoform
UniProt Gene Name
PITPNA
UniProt Synonym Gene Names
PITPN; PI-TP-alpha; PtdIns transfer protein alpha; PtdInsTP alpha
UniProt Entry Name
PIPNA_HUMAN
NCBI Description
This gene encodes a member of a family of lipid-binding proteins that transfer molecules of phosphatidylinositol or phosphatidylcholine between membrane surfaces. The protein is implicated in phospholipase C signaling and in the production of phosphatidylinositol 3,4,5-trisphosphate (PIP3) by phosphoinositide-3-kinase.[provided by RefSeq, Sep 2009]
Uniprot Description
PITPNA: Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes. Belongs to the PtdIns transfer protein family. PI transfer class I subfamily.
Protein type: Lipid-binding
Chromosomal Location of Human Ortholog: 17p13.3
Cellular Component: cytoplasm; myelin sheath
Molecular Function: lipid binding; phosphatidylcholine transmembrane transporter activity; phosphatidylinositol transporter activity
Biological Process: lipid metabolic process; phospholipid transport; visual perception
Research Articles on PITPN
1. This protein has been found differentially expressed in thalami from patients with schizophrenia.
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Product Notes
The PITPN pitpna (Catalog #AAA420567) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PITPN/PITP alpha Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's PITPN/PITP alpha can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 32kDa band observed in 293 and Human Liver lysates (predicted MW of 31kDa according to NP_006215). Recommended for use at 1-3ug/ml. Researchers should empirically determine the suitability of the PITPN pitpna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RQKDPVKGMT ADD. It is sometimes possible for the material contained within the vial of "PITPN/PITP alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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