Rabbit anti-Human, Mouse PISD Polyclonal Antibody | anti-PISD antibody

PISD, ID (Phosphatidylserine Decarboxylase Proenzyme, Phosphatidylserine Decarboxylase alpha Chain, Phosphatidylserine Decarboxylase beta Chain)

Cat. Num. AAA624991

• Gene Names: PISD; PSD; PSDC; PSSC; DJ858B16; dJ858B16.2
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry
• Purity: Purified by Protein A and Peptide affinity chromatography.
• Synonyms: PISD; Polyclonal Antibody; ID (Phosphatidylserine Decarboxylase Proenzyme; Phosphatidylserine Decarboxylase alpha Chain; Phosphatidylserine Decarboxylase beta Chain); Anti -PISD; anti-PISD antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PISD. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A and Peptide affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.
• Concentration: 0.51mg/ml (varies by lot)

• Applicable Applications for anti-PISD antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC). Other applications not tested.
• Application Notes: Recommended Dilution:; Immunohistochemistry (FFPE): 1:50-1:100; WB:1:1000; FC: 1:1-10-1:50; Optimal dilutions to be determined by the researcher.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 223-250 in the C-terminal region of human PISD.
• Localization: Mitochondrion
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis in mouse cerebellum tissue and mouse NIH-3T3 cell line lysates (35ug/lane) using MBS624991. PISD (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human brain using MBS624991, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NCI-H460 cells using MBS624991 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PISD antibody

Phosphatidylserine decarboxylases catalyze the formation of phosphatidylethanolamine (PE) by decarboxylation of phosphatidylserine (PS). Type I PSDs, such as PISD, are targeted to the inner mitochondrial membrane by an N-terminal targeting sequence. PISD also contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits

Product Categories/Family for anti-PISD antibody

Antibodies; Abs to Enzymes; Decarboxylase

NCBI and Uniprot Product Information

• NCBI GI #: 13489112
• NCBI GeneID: 23761
• NCBI Accession #: NP_055153
• NCBI GenBank Nucleotide #: NM_014338.3
• UniProt Accession #: Q9UG56; O43207; O95535; Q6IC28; Q96GQ2; Q9UGA9; B1AKM7
• Molecular Weight: 46,672 Da
• NCBI Official Full Name: phosphatidylserine decarboxylase proenzyme
• NCBI Official Synonym Full Names: phosphatidylserine decarboxylase
• NCBI Official Symbol: PISD
• NCBI Official Synonym Symbols: PSD; PSDC; PSSC; DJ858B16; dJ858B16.2
• NCBI Protein Information: phosphatidylserine decarboxylase proenzyme
• UniProt Protein Name: Phosphatidylserine decarboxylase proenzyme
• UniProt Gene Name: PISD
• UniProt Entry Name: PISD_HUMAN

NCBI Description

Phosphatidylserine decarboxylases (PSDs; EC 4.1.1.65) catalyze the formation of phosphatidylethanolamine (PE) by decarboxylation of phosphatidylserine (PS). Type I PSDs, such as PISD, are targeted to the inner mitochondrial membrane by an N-terminal targeting sequence. PISD also contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits (Schuiki and Daum, 2009 [PubMed 19165886]).[supplied by OMIM, Jul 2010]

Uniprot Description

PISD: Belongs to the phosphatidylserine decarboxylase family. Heterodimer. 2 isoforms of the human protein are produced by alternative splicing

Protein type: Endoplasmic reticulum; Lipid Metabolism - glycerophospholipid; Lyase; EC 4.1.1.65

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: mitochondrion; nucleus

Molecular Function: phosphatidylserine decarboxylase activity

Biological Process: phospholipid metabolic process; phosphatidylethanolamine biosynthetic process; glycerophospholipid biosynthetic process