Rabbit anti-Human, Mouse PISD Polyclonal Antibody | anti-PISD antibody

PISD Antibody

Cat. Num. AAA9631324

• Gene Names: PISD; PSD; PSDC; PSSC; DJ858B16; dJ858B16.2
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PISD; Polyclonal Antibody; PISD Antibody; DJ858B16; dJ858B16.2; DKFZp566G2246; Phosphatidylserine decarboxylase; Phosphatidylserine decarboxylase beta chain; Phosphatidylserine decarboxylase proenzyme; PISD_HUMAN; PSD; PSDC; PSSC; anti-PISD antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: PISD Antibody detects endogenous levels of PISD.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PISD antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human PISD.
• Conjugation: Unconjugated
• Similarity: Belongs to the phosphatidylserine decarboxylase family. PSD-B subfamily. Eukaryotic type I sub-subfamily.
• Post Translational Modifications: Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The autoendoproteolytic cleavage occurs by a canonical serine protease mechanism, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain. During this reaction, the Ser that is part of the protease active site of the proenzyme becomes the pyruvoyl prosthetic group, which constitutes an essential element of the active site of the mature decarboxylase.
• Subunit Structure: Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit.
• Function: Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine.
• Note: Anchored to the mitochondrial inner membrane through its interaction with the integral membrane beta chain.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-PISD antibody

Mitochondrion inner membrane; Single-pass membrane protein; Intermembrane side; Peripheral membrane protein; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 311033492
• NCBI GeneID: 23761
• UniProt Accession #: Q9UG56; O43207; O95535; Q6IC28; Q96GQ2; Q9UGA9; B1AKM7
• Molecular Weight: 409
• NCBI Official Full Name: Phosphatidylserine decarboxylase proenzyme
• NCBI Official Synonym Full Names: phosphatidylserine decarboxylase
• NCBI Official Symbol: PISD
• NCBI Official Synonym Symbols: PSD; PSDC; PSSC; DJ858B16; dJ858B16.2
• NCBI Protein Information: phosphatidylserine decarboxylase proenzyme
• UniProt Protein Name: Phosphatidylserine decarboxylase proenzyme
• UniProt Gene Name: PISD
• UniProt Entry Name: PISD_HUMAN

NCBI Description

Phosphatidylserine decarboxylases (PSDs; EC 4.1.1.65) catalyze the formation of phosphatidylethanolamine (PE) by decarboxylation of phosphatidylserine (PS). Type I PSDs, such as PISD, are targeted to the inner mitochondrial membrane by an N-terminal targeting sequence. PISD also contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits (Schuiki and Daum, 2009 [PubMed 19165886]).[supplied by OMIM, Jul 2010]

Uniprot Description

PISD: Belongs to the phosphatidylserine decarboxylase family. Heterodimer. 2 isoforms of the human protein are produced by alternative splicing

Protein type: Endoplasmic reticulum; Lipid Metabolism - glycerophospholipid; Lyase; EC 4.1.1.65

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: mitochondrion; nucleus

Molecular Function: phosphatidylserine decarboxylase activity

Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidylethanolamine biosynthetic process