Rabbit anti-Human PIP5KI gamma Polyclonal Antibody | anti-PIP5KIgamma antibody

PIP5KI gamma, CT (Phosphatidylinositol-4-phosphate 5-kinase, Type I, gamma, PtdIns(4)P-5-kinase 1 gamma, Phosphatidylinositol-4-phosphate 5-kinase Type I gamma, PIP5KIgamma, PIP5K1C, KIAA0589) (AP)

Cat. Num. AAA6493023

• Gene Names: PIP5K1C; LCCS3; PIP5Kgamma; PIP5K-GAMMA; PIP5K1-gamma
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PIP5KI gamma; Polyclonal Antibody; CT (Phosphatidylinositol-4-phosphate 5-kinase; Type I; gamma; PtdIns(4)P-5-kinase 1 gamma; Phosphatidylinositol-4-phosphate 5-kinase Type I gamma; PIP5KIgamma; PIP5K1C; KIAA0589) (AP); anti-PIP5KIgamma antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PIP5K1G.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 668

• Applicable Applications for anti-PIP5KIgamma antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PIP5K1G (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of hPIP5K1G-L652 in Hela cell line lysates (35ug/lane).PIP5K1G (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of PIP5K1C (arrow) using PIP5K1G Antibody (C-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIP5K1C gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-PIP5KIgamma antibody

Antibodies; Protein Kinases

References

1. Ling, K., et al., Nature 420(6911):89-93 (2002). 2. Di Paolo, G., et al., Nature 420(6911):85-89 (2002). 3. Ishihara, H., et al., J. Biol. Chem. 273(15):8741-8748 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 31317309
• NCBI GeneID: 23396
• NCBI Accession #: NP_036530
• NCBI GenBank Nucleotide #: NP_036530.1
• UniProt Accession #: O60331
• Molecular Weight: 73.26
• NCBI Official Full Name: phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform 2
• NCBI Official Synonym Full Names: phosphatidylinositol-4-phosphate 5-kinase type 1 gamma
• NCBI Official Symbol: PIP5K1C
• NCBI Official Synonym Symbols: LCCS3; PIP5Kgamma; PIP5K-GAMMA; PIP5K1-gamma
• NCBI Protein Information: phosphatidylinositol 4-phosphate 5-kinase type-1 gamma
• UniProt Protein Name: Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma
• UniProt Gene Name: PIP5K1C
• UniProt Synonym Gene Names: KIAA0589; PIP5K1-gamma; PtdIns(4)P-5-kinase 1 gamma; PIP5KIgamma
• UniProt Entry Name: PI51C_HUMAN

NCBI Description

This locus encodes a type I phosphatidylinositol 4-phosphate 5-kinase. The encoded protein catalyzes phosphorylation of phosphatidylinositol 4-phosphate, producing phosphatidylinositol 4,5-bisphosphate. This enzyme is found at synapses and has been found to play roles in endocytosis and cell migration. Mutations at this locus have been associated with lethal congenital contractural syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Sep 2010]

Uniprot Description

PIP5K1C: a member of the type I phosphatidylinositol-4-phosphate 5-kinase family of enzymes. A similar protein in mice is found in synapses and focal adhesion plaques, and binds the FERM domain of talin through its C-terminus.

Protein type: Kinase, lipid; Motility/polarity/chemotaxis; EC 2.7.1.68; Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: focal adhesion; endomembrane system; uropod; cytosol; nucleus; phagocytic cup

Molecular Function: protein binding; 1-phosphatidylinositol-4-phosphate 5-kinase activity; ATP binding

Biological Process: neutrophil chemotaxis; axon guidance; synaptic vesicle exocytosis; cell-cell adhesion; phosphoinositide phosphorylation; cytoskeletal anchoring; phospholipid metabolic process; synaptic vesicle endocytosis; phosphatidylinositol biosynthetic process; actin cytoskeleton organization and biogenesis; phagocytosis

Disease: Lethal Congenital Contracture Syndrome 3