Rabbit anti-Human, Mouse PINK1 Polyclonal Antibody | anti-PINK1 antibody

PINK1, NT (T133) (PARK6, Serine/threonine-protein kinase PINK1, mitochondrial, BRPK, PTEN-induced putative kinase protein 1) (PE)

Cat. Num. AAA6334189

• Gene Names: PINK1; BRPK; PARK6
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: PINK1; Polyclonal Antibody; NT (T133) (PARK6; Serine/threonine-protein kinase PINK1; mitochondrial; BRPK; PTEN-induced putative kinase protein 1) (PE); PARK6; PTEN-induced putative kinase protein 1; anti-PINK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PINK1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PINK1 (PARK6) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125~155 amino acids from the N-terminal region of human Park6 (PINK1).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Park6(PINK1) (arrow) using rabbit polyclonal Park6(PINK1) Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Park6(PINK1) gene.)

Western Blot (WB)

(Park6 (PINK1) Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Park6 (PINK1) antibody detected the Park6 (PINK1) protein (arrow).)

Western Blot (WB)

(Western blot analysis of anti-PARK6 Pab in mouse kidney tissue lysate. PARK6 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-PINK1 antibody

Defects in PINK1 are the cause of autosomal recessive early-onset Parkinson's disease 6 (PARK6). Six novel pathogenic PINK1 mutations suggest that PINK1 may be the second most common causative gene next to parkin in parkinsonism with the recessive mode of inheritance. Strong evidence indicates that, although important in mendelian forms of Parkinson's disease (PD), PINK1 does not influence the cause of sporadic nonmendelian forms of PD.

Product Categories/Family for anti-PINK1 antibody

Antibodies; Protein Kinases

References

Rogaeva, E., et al. Arch. Neurol. 61 (12), 1898-1904 (2004). Hatano, Y., et al. Ann. Neurol. 56 (3), 424-427 (2004). Healy, D.G., et al. Ann. Neurol. 56 (3), 329-335 (2004). Valente, E.M., et al. Science 304 (5674), 1158-1160 (2004). Nakajima, A., et al. Cancer Lett. 201 (2), 195-201 (2003). Unoki, M. and Nakamura, Y. Oncogene 20 (33), 4457-4465 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 14165272
• NCBI GeneID: 65018
• NCBI Accession #: NP_115785
• NCBI GenBank Nucleotide #: NM_032409.2
• UniProt Accession #: Q9BXM7; Q8N6T9; Q8NBU3; Q96DE4
• Molecular Weight: Predicted: 55 kDa; ; Observed: 53 kDa
• NCBI Official Full Name: serine/threonine-protein kinase PINK1, mitochondrial
• NCBI Official Synonym Full Names: PTEN induced putative kinase 1
• NCBI Official Symbol: PINK1
• NCBI Official Synonym Symbols: BRPK; PARK6
• NCBI Protein Information: serine/threonine-protein kinase PINK1, mitochondrial; protein kinase BRPK; PTEN-induced putative kinase protein 1
• UniProt Protein Name: Serine/threonine-protein kinase PINK1, mitochondrial
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PINK1
• UniProt Entry Name: PINK1_HUMAN

NCBI Description

This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq, Jul 2008]

Uniprot Description

PINK1: Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitment to dysfunctional mitochondria to initiate their degradation. Interats with PARK2. Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Membrane protein, integral; Protein kinase, Ser/Thr (non-receptor); Mitochondrial; EC 2.7.11.1; Protein kinase, Other; Other group; NKF2 family

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: mitochondrion; mitochondrial intermembrane space; cytosol; mitochondrial outer membrane; cytoskeleton; membrane; axon; perinuclear region of cytoplasm; cytoplasm; mitochondrial inner membrane; TORC2 complex; chromatin; nucleus; integral to mitochondrial outer membrane

Molecular Function: protein kinase B binding; protein serine/threonine kinase activity; protein binding; protease activator activity; protease binding; ubiquitin protein ligase binding; magnesium ion binding; kinase activity; ATP binding

Biological Process: ubiquitin-dependent protein catabolic process; negative regulation of JNK cascade; positive regulation of dopamine secretion; positive regulation of translation; regulation of protein ubiquitination; protein ubiquitination; protein amino acid phosphorylation; positive regulation of protein amino acid dephosphorylation; negative regulation of macroautophagy; regulation of mitochondrial membrane potential; positive regulation of ubiquitin-protein ligase activity; response to stress; regulation of protein complex assembly; negative regulation of neuron apoptosis; regulation of hydrogen peroxide metabolic process; mitochondrion organization and biogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein stabilization; activation of protein kinase B; positive regulation of synaptic transmission, dopaminergic; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; mitochondrion degradation; positive regulation of transcription factor activity; positive regulation of protein amino acid phosphorylation; response to oxidative stress; phosphorylation

Disease: Parkinson Disease 6, Autosomal Recessive Early-onset