Rabbit anti-Human, Mouse Pim1 Polyclonal Antibody | anti-Pim1 antibody

Pim1, CT (Pim1, Pim-1, Serine/threonine-protein kinase pim-1) (APC)

Cat. Num. AAA6334114

• Gene Names: Pim1; Pim-1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Pim1; Polyclonal Antibody; CT (Pim1; Pim-1; Serine/threonine-protein kinase pim-1) (APC); Serine/threonine-protein kinase pim-1; anti-Pim1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-Pim1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Pim1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-372 amino acids from the C-terminal region of Mouse Pim1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Pim1 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the Pim1 antibody detected the Pim1 protein (arrow).)

Western Blot (WB)

(Mouse Pim1 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Pim1 antibody detected the Pim1 protein (arrow).)

Related Product Information for anti-Pim1 antibody

Pim1 may affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3 (By similarity). Promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B (By similarity). Also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3 (By similarity). Plays a role in signal transduction in blood cells (By similarity). Contributes to both cell proliferation and survival thus providing a selective advantage in tumorigenesis (By similarity).

Product Categories/Family for anti-Pim1 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 83305340
• NCBI GeneID: 18712
• UniProt Accession #: P06803
• Molecular Weight: 35,537 Da
• NCBI Official Full Name: Serine/threonine-protein kinase pim-1
• NCBI Official Synonym Full Names: proviral integration site 1
• NCBI Official Symbol: Pim1
• NCBI Official Synonym Symbols: Pim-1
• NCBI Protein Information: serine/threonine-protein kinase pim-1
• UniProt Protein Name: Serine/threonine-protein kinase pim-1
• Protein Family: Protein
• UniProt Gene Name: Pim1
• UniProt Synonym Gene Names: Pim-1

Uniprot Description

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, an other proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B,induces 14-3-3 binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis ().