Rabbit PIK3R6 Polyclonal Antibody | anti-PIK3R6 antibody

PIK3R6 Polyclonal Antibody

Cat. Num. AAA3006457

• Gene Names: PIK3R6; C17orf38; HsT41028; p87PIKAP; p84 PIKAP; p87(PIKAP)
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PIK3R6; Polyclonal Antibody; PIK3R6 Polyclonal Antibody; Phosphoinositide 3-kinase regulatory subunit 6; Phosphoinositide 3-kinase gamma adapter protein of 87 kDa; p84 PI3K adapter protein; p84 PIKAP; p87 PI3K adapter protein; p87PIKAP; C17orf38; anti-PIK3R6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PIK3R6 polyclonal antibody detects endogenous levels of PIK3R6 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 754

• Applicable Applications for anti-PIK3R6 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human PIK3R6.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PIK3R6 polyclonal antibody at 1:500 dilutionLane1:HepG2 whole cell lysateLane2:Mouse brain tissue lysateLane3:Rat brain tissue lysate)

Related Product Information for anti-PIK3R6 antibody

p87PIKAP (p87 PI3K adapter protein), also known as PIK3R6 (phosphoinositide-3-kinase, regulatory subunit 6), p84 or HsT41028, is a 754 amino acid cytoplasmic protein that functions as a regulatory subunit of the PI 3-kinase p110 complex. Expressed in heart, dendritic cells, macrophages and neutrophils, p87PIKAP drives PI 3-kinase p110© activation, interacts with PDE3B and is thought to cause the two proteins to form a scaffolding interaction. The gene encoding p87PIKAP maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1, 200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.

NCBI and Uniprot Product Information

• NCBI GI #: 58082081
• NCBI GeneID: 146850
• NCBI Accession #: NP_001010855.1
• NCBI GenBank Nucleotide #: NP_001010855.1
• UniProt Accession #: Q5UE93
• Molecular Weight: ~ 84kDa
• NCBI Official Full Name: phosphoinositide 3-kinase regulatory subunit 6 isoform 1
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase regulatory subunit 6
• NCBI Official Symbol: PIK3R6
• NCBI Official Synonym Symbols: C17orf38; HsT41028; p87PIKAP; p84 PIKAP; p87(PIKAP)
• NCBI Protein Information: phosphoinositide 3-kinase regulatory subunit 6
• UniProt Protein Name: Phosphoinositide 3-kinase regulatory subunit 6
• Protein Family: Phosphoinositide 3-kinase regulatory
• UniProt Gene Name: PIK3R6
• UniProt Synonym Gene Names: C17orf38; p84 PIKAP; p87PIKAP

NCBI Description

Phosphoinositide 3-kinase gamma is a lipid kinase that produces the lipid second messenger phosphatidylinositol 3,4,5-trisphosphate. The kinase is composed of a catalytic subunit and one of several regulatory subunits, and is chiefly activated by G protein-coupled receptors. This gene encodes a regulatory subunit, and is distantly related to the phosphoinositide-3-kinase, regulatory subunit 5 gene which is located adjacent to this gene on chromosome 7. The orthologous protein in the mouse binds to both the catalytic subunit and to G(beta/gamma), and mediates activation of the kinase subunit downstream of G protein-coupled receptors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

PIK3R6: Regulatory subunit of the PI3K gamma complex. Acts as an adapter to drive activation of PIK3CG by beta-gamma G protein dimers. The PIK3CG:PIK3R6 heterodimer is much less sensitive to beta-gamma G protein dimers than PIK3CG:PIK3R5 and its membrane recruitment and beta-gamma G protein dimer-dependent activation requires HRAS1 bound to PIK3CG. Recruits of the PI3K gamma complex to a PDE3B:RAPGEF3 signaling complex involved in angiogenesis; signaling seems to involve RRAS.

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytosol; membrane; phosphoinositide 3-kinase complex; plasma membrane

Molecular Function: 1-phosphatidylinositol-3-kinase regulator activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding

Biological Process: angiogenesis; G-protein coupled receptor signaling pathway; phosphatidylinositol biosynthetic process; phosphatidylinositol phosphorylation; phosphoinositide 3-kinase cascade; platelet activation; positive regulation of angiogenesis; positive regulation of MAP kinase activity; positive regulation of T cell differentiation; regulation of natural killer cell mediated cytotoxicity; regulation of phosphoinositide 3-kinase activity