Rabbit anti-Human PIK3R5 Polyclonal Antibody | anti-PIK3R5 antibody

PIK3R5, CT (PIK3R5, Phosphoinositide 3-kinase regulatory subunit 5, PI3-kinase p101 subunit, Phosphatidylinositol 4,5-bisphosphate 3-kinase regulatory subunit, Protein FOAP-2, PtdIns-3-kinase p101, p101-PI3K) (PE)

Cat. Num. AAA6334112

• Gene Names: PIK3R5; p101; FOAP-2; P101-PI3K; F730038I15Rik
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PIK3R5; Polyclonal Antibody; CT (PIK3R5; Phosphoinositide 3-kinase regulatory subunit 5; PI3-kinase p101 subunit; Phosphatidylinositol 4; 5-bisphosphate 3-kinase regulatory subunit; Protein FOAP-2; PtdIns-3-kinase p101; p101-PI3K) (PE); p101-PI3K; anti-PIK3R5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PIK3R5.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PIK3R5 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 763-792 in the C-terminal region of human PIK3R5.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in WiDr cell line lysates (35ug/lane) usingMBS642665. This demonstrates that MBS642665 detected the PIK3R5 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue using MBS642665 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS642665 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of WiDr cells using MBS642665 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PIK3R5 antibody

Antibodies; Protein Kinases

References

1. Rose, J. Phd, et al. Mol. Med. (2010). In press: 2. Segat, L., et al. Vaccine 28(10):2201-2206(2010). 3. Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009). 4. Johnson, C., et al. Oncogene 26(49):7049-7057(2007). 5. Suire, S., et al. Curr. Biol. 15(6):566-570(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 217330639
• NCBI GeneID: 23533
• NCBI Accession #: NP_001136105.1; NP_055123.2
• NCBI GenBank Nucleotide #: NM_001142633.2
• UniProt Accession #: Q8WYR1; Q5G936; Q5G938; Q5G939; Q8IZ23; Q9Y2Y2; B0LPH4; D3DTS3
• Molecular Weight: 97,348 Da
• NCBI Official Full Name: phosphoinositide 3-kinase regulatory subunit 5 isoform 1
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, regulatory subunit 5
• NCBI Official Symbol: PIK3R5
• NCBI Official Synonym Symbols: p101; FOAP-2; P101-PI3K; F730038I15Rik
• NCBI Protein Information: phosphoinositide 3-kinase regulatory subunit 5; protein FOAP-2; ptdIns-3-kinase p101; PI3-kinase p101 subunit; phosphatidylinositol 4,5-bisphosphate 3-kinase regulatory subunit; phosphatidylinositol-4,5-bisphosphate 3-kinase regulatory subunit
• UniProt Protein Name: Phosphoinositide 3-kinase regulatory subunit 5
• Protein Family: Phosphoinositide 3-kinase regulatory
• UniProt Gene Name: PIK3R5
• UniProt Synonym Gene Names: PI3-kinase regulatory subunit 5
• UniProt Entry Name: PI3R5_HUMAN

NCBI Description

Phosphatidylinositol 3-kinases (PI3Ks) phosphorylate the inositol ring of phosphatidylinositol at the 3-prime position, and play important roles in cell growth, proliferation, differentiation, motility, survival and intracellular trafficking. The PI3Ks are divided into three classes: I, II and III, and only the class I PI3Ks are involved in oncogenesis. This gene encodes the 101 kD regulatory subunit of the class I PI3K gamma complex, which is a dimeric enzyme, consisting of a 110 kD catalytic subunit gamma and a regulatory subunit of either 55, 87 or 101 kD. This protein recruits the catalytic subunit from the cytosol to the plasma membrane through high-affinity interaction with G-beta-gamma proteins. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found. [provided by RefSeq, Oct 2011]

Uniprot Description

PIK3R5: Regulatory subunit of the PI3K gamma complex. Heterodimer of a catalytic subunit (PIK3CG/p120) and a regulatory (PIK3R5a/p101) subunit. Interacts with G beta gamma proteins. Ubiquitously expressed with high expression in fetal brain compared to adult brain. Abundant expression is observed in cerebellum, cerebral cortex, cerebral meninges, and vermis cerebelli. Greatly activated by G gamma proteins. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: membrane; cytoplasm; cytosol; nucleus

Molecular Function: G-protein beta/gamma-subunit binding; 1-phosphatidylinositol-3-kinase regulator activity

Biological Process: positive regulation of MAP kinase activity; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; platelet activation; phosphoinositide 3-kinase cascade; phospholipid metabolic process; phosphatidylinositol biosynthetic process; blood coagulation; regulation of phosphoinositide 3-kinase activity

Disease: Ataxia-oculomotor Apraxia 3