Rabbit PIK3R4 Polyclonal Antibody | anti-PIK3R4 antibody

PIK3R4 Antibody

Cat. Num. AAA9608785

• Gene Names: PIK3R4; p150; VPS15
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: PIK3R4; Polyclonal Antibody; PIK3R4 Antibody; MGC102700; P150; Phosphatidylinositol 3 kinase associated p150; Phosphatidylinositol 3 kinase regulatory subunit polypeptide 4; Phosphatidylinositol 3 kinase regulatory subunit polypeptide 4 p150; Phosphoinositide 3 kinase adaptor protein; Phosphoinositide 3 kinase regulatory subunit 4; Phosphoinositide 3 kinase regulatory subunit 4 p150; Phosphoinositide 3 kinase regulatory subunit polypeptide 4; Phosphoinositide 3 kinase regulatory subunit polypeptide 4 p150; Phosphoinositide 3-kinase adaptor protein; Phosphoinositide 3-kinase regulatory subunit 4; PI3 kinase p150 subunit; PI3-kinase p150 subunit; PI3-kinase regulatory subunit 4; PI3R4_HUMAN; PIK3R 4; VPS 15; VPS15; anti-PIK3R4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PIK3R4 antibody detects endogenous levels of total PIK3R4
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1358

• Applicable Applications for anti-PIK3R4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human PIK3R4
• Subcellular Location: Endosome
• Tissue Specificity: Ubiquitously expressed.
• Predicted Cross Reactivity: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Xenopus
• Similarity: Pig (92%), Zebrafish (83%), Bovine (92%), Horse (100%), Rabbit (92%), Dog (92%), Xenopus (83%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of PIK3R4 using COLO205 whole cell lysates)

Related Product Information for anti-PIK3R4 antibody

Function: Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123).
Subunit Structure: Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex the core of which is composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 associating with additional regulatory/auxilliary subunits to form alternative complex forms. Alternative complex forms containing a forth regulatory subunit in a mutually exclusive manner are PI3K complex I (PI3KC3-C1) containing ATG14, and PI3K complex II (PI3KC3-C2) containing UVRAG (PubMed:8999962, PubMed:19270696, PubMed:23878393, PubMed:25490155). PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex (PubMed:25490155). Both, PI3KC3-C1 and PI3KC3-C2, can associate with further regulatory subunits, such as RUBCN, SH3GLB1/Bif-1, AMBRA1 and NRBF2 (PubMed:19270696, PubMed:20643123, PubMed:24785657). PI3KC3-C1 probably associates with PIK3CB (By similarity). Interacts with RAB7A in the presence of PIK3C3/VPS34 (PubMed:14617358). Interacts with NRBF2 (PubMed:24785657).
Post-translational Modifications: Myristoylated. Probably autophosphorylated.
Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

NCBI and Uniprot Product Information

• NCBI GI #: 23943912
• NCBI GeneID: 30849
• NCBI Accession #: NP_055417.1
• NCBI GenBank Nucleotide #: NM_014602.2
• UniProt Accession #: Q99570; Q2TBF4
• Molecular Weight: Observed: 153 kDa; Predicted: 154 kDa
• NCBI Official Full Name: phosphoinositide 3-kinase regulatory subunit 4
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase regulatory subunit 4
• NCBI Official Symbol: PIK3R4
• NCBI Official Synonym Symbols: p150; VPS15
• NCBI Protein Information: phosphoinositide 3-kinase regulatory subunit 4
• UniProt Protein Name: Phosphoinositide 3-kinase regulatory subunit 4
• Protein Family: Phosphoinositide 3-kinase regulatory
• UniProt Gene Name: PIK3R4
• UniProt Synonym Gene Names: PI3-kinase regulatory subunit 4

Uniprot Description

Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123).