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Western Blot (WB) (Western blot analysis of 50 ug of whole cell lysate (A: Mouse liver) using a 5% SDS PAGE gel and PIK3R4 antibody at a dilution of 1:500)

Rabbit PIK3R4 Polyclonal Antibody | anti-PIK3R4 antibody

PIK3R4 Antibody

Gene Names
PIK3R4; p150; VPS15
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
PIK3R4; Polyclonal Antibody; PIK3R4 Antibody; Affinity Purified Rabbit Polyclonal PIK3R4 Antibody; Polyclonal PIK3R4 antibody; Anti-PIK3R4 antibody; PIKR4 3 antibody; PI3-kinase p150 subunit antibody; phosphatidylinositol 3-kinase-associated p150 antibody; phosphoinositide 3-kinase adaptor protein antibody; phosphoinositide-3-kinase regulatory subunit 4 p150 antibody; PIKR4 3; p150 antibody; PIKR4-3 antibody; MGC102700 antibody; VPS15 antibody; PIK3R4 antibody; PIKR4-3; PI3-kinase regulatory subunit 4 antibody; anti-PIK3R4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.8 mg/ml (varies by lot)
Sequence Length
1358
Applicable Applications for anti-PIK3R4 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF: 1:100-1:500
ICC: 1:100-1:500
IHC-P: 1:100-1:500
WB: 1:500-1:3000
Immunogen
PIK3R4 antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 341 of PIK3R4
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of 50 ug of whole cell lysate (A: Mouse liver) using a 5% SDS PAGE gel and PIK3R4 antibody at a dilution of 1:500)

Western Blot (WB) (Western blot analysis of 50 ug of whole cell lysate (A: Mouse liver) using a 5% SDS PAGE gel and PIK3R4 antibody at a dilution of 1:500)

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A: A431) using a 5% SDS PAGE gel and PIK3R4 antibody at a dilution of 1:1000)

Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A: A431) using a 5% SDS PAGE gel and PIK3R4 antibody at a dilution of 1:1000)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using p150(PIK3R4) antibody at 1:200 dilution.)

Immunofluorescence (IF) (Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using p150(PIK3R4) antibody at 1:200 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of paraffin-embedded SAS xenograft using PIK3R4 antibody at a dilution of 1:500)

Immunohistochemistry (IHC) (Immunohistochemical staining of paraffin-embedded SAS xenograft using PIK3R4 antibody at a dilution of 1:500)
Related Product Information for anti-PIK3R4 antibody
Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.
Product Categories/Family for anti-PIK3R4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
153 kDa
NCBI Official Full Name
phosphoinositide 3-kinase regulatory subunit 4
NCBI Official Synonym Full Names
phosphoinositide-3-kinase regulatory subunit 4
NCBI Official Symbol
PIK3R4
NCBI Official Synonym Symbols
p150; VPS15
NCBI Protein Information
phosphoinositide 3-kinase regulatory subunit 4
UniProt Protein Name
Phosphoinositide 3-kinase regulatory subunit 4
UniProt Gene Name
PIK3R4
UniProt Synonym Gene Names
PI3-kinase regulatory subunit 4
UniProt Entry Name
PI3R4_HUMAN

Uniprot Description

PIK3R4: Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Kinase, lipid; Protein kinase, Other; Other group; VPS15 family

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: membrane; late endosome; nuclear pore; axoneme; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: macroautophagy; phospholipid metabolic process; late endosome to vacuole transport; toll-like receptor signaling pathway; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; innate immune response; protein targeting to vacuole; toll-like receptor 9 signaling pathway; peroxisome degradation; protein amino acid phosphorylation

Research Articles on PIK3R4

Similar Products

Product Notes

The PIK3R4 pik3r4 (Catalog #AAA5312151) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PIK3R4 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF: 1:100-1:500 ICC: 1:100-1:500 IHC-P: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the PIK3R4 pik3r4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIK3R4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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