Rabbit PIK3C3 Polyclonal Antibody | anti-PIK3C3 antibody

PIK3C3 Antibody

Cat. Num. AAA153658

• Gene Names: PIK3C3; VPS34; hVps34
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: PIK3C3 antibody is affinity chromatography purified via peptide column.
• Synonyms: PIK3C3; Polyclonal Antibody; PIK3C3 Antibody; VPS34; hVps34; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; PI3-kinase type 3; phosphatidylinositol 3-kinase; catalytic subunit type 3; anti-PIK3C3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PIK3C3 antibody is human, mouse and rat reactive.
• Purity/Purification: PIK3C3 antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 887

• Applicable Applications for anti-PIK3C3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: PIK3C3 antibody can be used for detection of PIK3C3 by Western blot at 1 - 2 mug/ml.
• Conjugate: Unconjugated
• Immunogen: PIK3C3 antibody was raised against an 18 amino acid peptide near the amino terminus of human PIK3C3.
• Buffer: PIK3C3 antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: PIK3C3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of PIK3C3 in mouse small intestine tissue lysate with PIK3C3 antibody at 1 μg/ml.)

Related Product Information for anti-PIK3C3 antibody

PI 3-kinase p100 (phosphoinositide-3-kinase p100 subunit), also known as hVps34 or PIK3C3 (phosphoinositide-3-kinase class III), is a member of the PI3/PI4-kinase family (1). It is a catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate and ubiquitously expressed with a highest expression in skeletal muscle (1,2). PIK3C3 is involved in the endosome to lysosome transport and plays important roles in intracellular membrane trafficking and autophagy (3-5).

NCBI and Uniprot Product Information

• NCBI GI #: 34761064
• NCBI GeneID: 5289
• NCBI Accession #: NP_002638
• NCBI GenBank Nucleotide #: NM_002647.2
• UniProt Accession #: Q8NEB9; Q15134
• Molecular Weight: Predicted: 91; 98 kDa; ; Observed: 98 kDa
• NCBI Official Full Name: phosphatidylinositol 3-kinase catalytic subunit type 3
• NCBI Official Synonym Full Names: phosphatidylinositol 3-kinase, catalytic subunit type 3
• NCBI Official Symbol: PIK3C3
• NCBI Official Synonym Symbols: VPS34; hVps34
• NCBI Protein Information: phosphatidylinositol 3-kinase catalytic subunit type 3; PI3K type 3; PI3-kinase type 3; PtdIns-3-kinase type 3; phosphoinositide-3-kinase, class 3; phosphatidylinositol 3-kinase p100 subunit
• UniProt Protein Name: Phosphatidylinositol 3-kinase catalytic subunit type 3
• UniProt Gene Name: PIK3C3
• UniProt Synonym Gene Names: VPS34; PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3
• UniProt Entry Name: PK3C3_HUMAN

Uniprot Description

PIK3C3: Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes. Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4. Forms a complex with BECN1, PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. In this complex, presence of UVRAG and ATG14 are mutually exclusive. Part of a complex composed of PIK3R4 and PIK3CB. Interacts with RAB7A in the presence of PIK3R4. Ubiquitously expressed, with a highest expression in skeletal muscle. Belongs to the PI3/PI4-kinase family.

Protein type: Motility/polarity/chemotaxis; Kinase, lipid; Carbohydrate Metabolism - inositol phosphate; Autophagy; EC 2.7.1.137

Chromosomal Location of Human Ortholog: 18q12.3

Cellular Component: membrane; late endosome; phagocytic vesicle; axoneme; midbody; cytosol; phosphoinositide 3-kinase complex

Molecular Function: protein binding; 1-phosphatidylinositol-3-kinase activity; ATP binding; protein kinase activity

Biological Process: phosphoinositide-mediated signaling; endosome organization and biogenesis; cytokinesis; protein amino acid phosphorylation; response to leucine; regulation of protein secretion; early endosome to late endosome transport; phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process; toll-like receptor signaling pathway; insulin receptor signaling pathway; innate immune response; protein processing; toll-like receptor 9 signaling pathway; autophagic vacuole formation