Rabbit anti-Human HTR2C Polyclonal Antibody | anti-HTR2C antibody

HTR2C, ID (HTR2C, HTR1C, 5-hydroxytryptamine receptor 2C, 5-hydroxytryptamine receptor 1C, Serotonin receptor 2C) (PE)

Cat. Num. AAA6308836

• Gene Names: HTR2C; HTR1C; 5-HT2C; 5-HTR2C
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HTR2C; Polyclonal Antibody; ID (HTR2C; HTR1C; 5-hydroxytryptamine receptor 2C; 5-hydroxytryptamine receptor 1C; Serotonin receptor 2C) (PE); Serotonin receptor 2C; anti-HTR2C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-HTR2C antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HTR2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 252-282 amino acids from the Central region of human HTR2C.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(HTR2C Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the HTR2C antibody detected the HTR2C protein (arrow).)

Related Product Information for anti-HTR2C antibody

Serotonin (5-hydroxytryptamine, 5-HT), a neurotransmitter, elicits a wide array of physiological effects by binding to several receptor subtypes, including the 5-HT2 family of seven-transmembrane-spanning, G-protein-coupled receptors, which activate phospholipase C and D signaling pathways. This gene encodes the 2C subtype of serotonin receptor and its mRNA is subject to multiple RNA editing events, where genomically encoded adenosine residues are converted to inosines. RNA editing is predicted to alter amino acids within the second intracellular loop of the 5-HT2C receptor and generate receptor isoforms that differ in their ability to interact with G proteins and the activation of phospholipase C and D signaling cascades, thus modulating serotonergic neurotransmission in the central nervous system. Studies in humans have reported abnormalities in patterns of 5-HT2C editing in depressed suicide victims.

Product Categories/Family for anti-HTR2C antibody

Antibodies; Receptors

References

Gregoor, J.G., et al. Psychiatr. Genet. 20(6):311-316(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Kiezebrink, K., et al. World J. Biol. Psychiatry 11(6):824-833(2010). Risselada, A.J., et al. Pharmacogenomics J. (2010). In press: McGrew, L., et al. Mol. Pharmacol. 65(1):252-256(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4504541
• NCBI GeneID: 3358
• NCBI Accession #: NP_000859.1
• NCBI GenBank Nucleotide #: NM_000868.2
• UniProt Accession #: P28335; Q5VUF8; Q9NP28; B1AMW4
• Molecular Weight: 51,821 Da
• NCBI Official Full Name: 5-hydroxytryptamine receptor 2C isoform a
• NCBI Official Synonym Full Names: 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled
• NCBI Official Symbol: HTR2C
• NCBI Official Synonym Symbols: HTR1C; 5-HT2C; 5-HTR2C
• NCBI Protein Information: 5-hydroxytryptamine receptor 2C; 5-HT1C; 5-HT-1C; 5-HT-2C; serotonin receptor 2C; serotonin 5-HT-2C receptor; 5-hydroxytryptamine receptor 1C; 5-hydroxytryptamine receptor-1c
• UniProt Protein Name: 5-hydroxytryptamine receptor 2C
• Protein Family: 5-hydroxytryptamine receptor
• UniProt Gene Name: HTR2C
• UniProt Synonym Gene Names: HTR1C; 5-HT-2C; 5-HT2C; 5-HTR2C; 5-HT-1C; 5-HT1C
• UniProt Entry Name: 5HT2C_HUMAN

NCBI Description

Serotonin (5-hydroxytryptamine, 5-HT), a neurotransmitter, elicits a wide array of physiological effects by binding to several receptor subtypes, including the 5-HT2 family of seven-transmembrane-spanning, G-protein-coupled receptors, which activate phospholipase C and D signaling pathways. This gene encodes the 2C subtype of serotonin receptor and its mRNA is subject to multiple RNA editing events, where genomically encoded adenosine residues are converted to inosines. RNA editing is predicted to alter amino acids within the second intracellular loop of the 5-HT2C receptor and generate receptor isoforms that differ in their ability to interact with G proteins and the activation of phospholipase C and D signaling cascades, thus modulating serotonergic neurotransmission in the central nervous system. Studies in humans have reported abnormalities in patterns of 5-HT2C editing in depressed suicide victims. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

5-HT(2C): a G-protein coupled receptor. One of several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. Mediates its action by activating a phosphatidylinositol-calcium second messenger system.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: serotonin binding; serotonin receptor activity; 5-HT2 receptor activity; drug binding

Biological Process: response to drug; cGMP biosynthetic process; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); behavioral fear response; regulation of corticotropin-releasing hormone secretion; serotonin receptor, phospholipase C activating pathway; locomotory behavior; positive regulation of phosphatidylinositol biosynthetic process; cellular calcium ion homeostasis; synaptic transmission; regulation of neurological process; release of sequestered calcium ion into cytosol; regulation of appetite; positive regulation of fat cell differentiation; feeding behavior; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); serotonin receptor signaling pathway