Goat PIK3C2A Polyclonal Antibody | anti-PIK3C2A antibody

Goat anti-PIK3C2A Antibody

Cat. Num. AAA420452

• Gene Names: PIK3C2A; CPK; PI3-K-C2A; PI3K-C2alpha; PI3K-C2-alpha; PI3-K-C2(ALPHA)
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Dog
• Applications: ELISA, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: PIK3C2A; Polyclonal Antibody; Goat anti-PIK3C2A Antibody; CPK; PI3-K-C2A; PI3K-C2alpha; PI3-K-C2(ALPHA); phosphoinositide-3-kinase; class 2; alpha polypeptide; C2-containing phosphatidylinositol kinase; DKFZp686L193; MGC142218; PIK3C2A antibody; CPK antibody; PI3-K-C2A antibody; PI3K-C2alpha antibody; PI3-K-C2(ALPHA) antibody; alpha polypeptide antibody; C2-containing phosphatidylinositol kinase antibody; DKFZp686L193 antibody; MGC142218 antibody; anti-PIK3C2A antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Dog
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: TVKWYQLTAATYL
• Sequence Length: 1686

• Applicable Applications for anti-PIK3C2A antibody: Peptide ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Immunohistochemistry: In paraffin embedded Human Heart shows strong staining of the capillary network and patterned staining of the myocardial fibres in transverse section. Recommended concentration, 5-10ug/ml.; Western Blot: Preliminary experiments gave no signal but low background in Human Testis and Molt-4 lysates at up to 1ug/ml.
• Immunogen: Peptide with sequence C-TVKWYQLTAATYL, from the C Terminus of the protein sequence according to NP_002636.2.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 157671929
• NCBI GeneID: 5286
• NCBI Accession #: NP_002636.2
• NCBI GenBank Nucleotide #: NM_002645.3
• Molecular Weight: 53,742 Da
• NCBI Official Full Name: phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha isoform 1
• NCBI Official Synonym Full Names: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
• NCBI Official Symbol: PIK3C2A
• NCBI Official Synonym Symbols: CPK; PI3-K-C2A; PI3K-C2alpha; PI3K-C2-alpha; PI3-K-C2(ALPHA)
• NCBI Protein Information: phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
• UniProt Protein Name: Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
• Protein Family: Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing
• UniProt Gene Name: PIK3C2A
• UniProt Synonym Gene Names: PI3K-C2-alpha; PtdIns-3-kinase C2 subunit alpha
• UniProt Entry Name: P3C2A_HUMAN

NCBI Description

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. [provided by RefSeq, Jul 2008]

Uniprot Description

PIK3C2A: a protein of the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. Its PI3-kinase is not sensitive to nanomolar levels of the inhibitor wortmannin. Activated by insulin and may be involved in integrin-dependent signaling.

Protein type: Autophagy; EC 2.7.1.154; Carbohydrate Metabolism - inositol phosphate; Motility/polarity/chemotaxis; Kinase, lipid

Chromosomal Location of Human Ortholog: 11p15.5-p14

Cellular Component: clathrin-coated vesicle; cytoplasm; cytosol; Golgi apparatus; membrane; nucleus; phosphoinositide 3-kinase complex; plasma membrane; vesicle

Molecular Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; phosphatidylinositol-4-phosphate 3-kinase activity; phosphoinositide 3-kinase activity; phosphoinositide binding

Biological Process: clathrin cage assembly; endocytosis; epidermal growth factor receptor signaling pathway; exocytosis; insulin receptor signaling pathway; phosphatidylinositol biosynthetic process; phosphoinositide phosphorylation; phosphoinositide-mediated signaling; platelet-derived growth factor receptor signaling pathway; vascular smooth muscle contraction